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二甲双胍对成骨细胞葡萄糖摄取及葡萄糖转运蛋白-1表达的影响 被引量:5

Effect of metformin on glucose uptake and glucose transporter-1 expression in osteoblasts
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摘要 目的:研究盐酸二甲双胍(MF)对大鼠下颌骨成骨细胞葡萄糖摄取及葡萄糖转运蛋白-1(GLUT-1)表达的影响。方法:分离培养原代下颌骨成骨细胞,分别给予不同浓度的MF作用24h,采用氟-18(F-18)标记的脱氧葡萄糖(18F-FDG)测定成骨细胞的葡萄糖摄取,GLUT-1表达水平的检测采用Western blot法。结果:MF显著刺激下颌骨成骨细胞对18F-FDG摄取,同时伴随GLUT-1表达的明显增加,其作用呈剂量依赖性效应。结论:MF促进成骨细胞对葡萄糖的摄取及GLUT-1的表达。 Objective: To investigate the effect of metformin on glucose uptake and GLUT-1 expression in osteoblasts from rat mandible. Methods: Primary osteoblasts were isolated and cultured. Cells were treated with metformin in different concentrations for 24h, GLUT-1 expression level was evaluated by Western blot analysis, and glucose uptake was detected by ^18F-FDG in the cells. Results: Metformin significantly promoted ^18F-FDG uptake, and increased GLUT-1 expression simultaneously dose-dependentlly. Conclusion: These results show that MF treatment promotes glucose uptake and upregulates expression of GLUT-1 in osteoblasts.
出处 《口腔颌面修复学杂志》 2008年第2期121-123,共3页 Chinese Journal of Prosthodontics
关键词 成骨细胞 盐酸二甲双胍 葡萄糖转运蛋白-1 葡萄糖摄取 osteoblasts metformin GLUT-1 glucose uptake
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参考文献15

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共引文献81

同被引文献142

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