摘要
Objective： Ultraviolet （UV） radiation is one of the important cataract risk factors, However, the pathogenesis is still poorly understood. The migration of human lens epithelial cells（HLECs） plays a Clucial role in the remodeling of lens capsule and cataract formation. The purpose of this study is to investigate the mechanism of UV inducing cataractogenesis. Methods：The toxicity of UV-irradiation on HLECs was assessed by Methyl thiazolyl tetrazolium（MTT） assay. The activity of matrix metalloproteinase-2（MMP-2） was observed by Gelatin zymography. The migration of HLECs was examined by Cell Track Motility. Results：UV-irradiation does great harm to HLECs, and may induce apoptosis in the cells when UV higher than 15 mj/cm2. UV significantly increased MMP-2 activity in a timedependent manner. In addition, the irradiation could induce the migration of HLECs. Conclusion：UV-irradiation could induce the migration of HLECs by increasing the activity of MMP-2.
Objective： Ultraviolet （UV） radiation is one of the important cataract risk factors, However, the pathogenesis is still poorly understood. The migration of human lens epithelial cells（HLECs） plays a Clucial role in the remodeling of lens capsule and cataract formation. The purpose of this study is to investigate the mechanism of UV inducing cataractogenesis. Methods：The toxicity of UV-irradiation on HLECs was assessed by Methyl thiazolyl tetrazolium（MTT） assay. The activity of matrix metalloproteinase-2（MMP-2） was observed by Gelatin zymography. The migration of HLECs was examined by Cell Track Motility. Results：UV-irradiation does great harm to HLECs, and may induce apoptosis in the cells when UV higher than 15 mj/cm2. UV significantly increased MMP-2 activity in a timedependent manner. In addition, the irradiation could induce the migration of HLECs. Conclusion：UV-irradiation could induce the migration of HLECs by increasing the activity of MMP-2.
基金
This work was supported by the project of the Natural Science Foun- dation of Jiangsu province(No.BK2007234)
the Institution Natural Science Foundation of Jiangsu province(No.07KJD320140)
