奶瓶中双酚A、烷基酚及烷基酚聚氧乙烯醚残留量的高效液相色谱-荧光测定法

Determination of Biphenol A,Alkylpheniols and Alkylphenol Ethoxylates Residues in Nursing Bottle by HPLC-RF

建立了奶瓶中双酚A、烷基酚及烷基酚聚氧乙烯醚残留量的高效液相色谱-荧光测定方法。样品用二氯甲烷-甲醇提取后,采用高效液相色谱分离,荧光检测器检测。BPA、OP、NP、OPEO在0.05～1.0μg/mL,NPEO在0.2～1.0μg/mL范围呈线性。对奶瓶样品作添加回收试验,各种成分的平均回收率在81.1%～100.3%之间,相对标准偏差在2.7%～5.5%之间。方法检出限:BPA为1mg/kg;OP、NP、OPEO为2mg/kg;NPEO为5mg/kg。结果表明,该法简便、灵敏、精确度高,适用于奶瓶中双酚A、烷基酚及烷基酚聚氧乙烯醚的残留分析。

A method was developed for the determination of biphenol A, alkylpheniols and alkylphenol ethoxylates residues in nursing bottle by HPLC-RF. Samples were extracted by dichloromethane- methanol, and analyzed by HPLC-RF. The linear range of BPA, OP, NP, OPEO were within 0.05-1.0 μg/mL and that of NPEO was within 0.2-1.0 μg/mL. Recovery rates of these compounds in nursing bottle ranged from 81.1% to 100.3%, and the relative standard deviations were of 2.7%-5,5%. The detection limits of the method was： BPA 1 mg/kg, OP 2 mg/kg, NP2 mg/kg, OPEO 2 mg/kg, and NPEO 5 mg/kg. This method was simple, sensitive and precise, which was suitable for qualitative and quantitative analysis ofBPA, AP and APEO residues in nursing bottle.