摘要
目的:为优选半夏白术天麻汤用半仿生-酶法提取的工艺条件,以甘草次酸为综合评判指标之一,探讨采用HPLC法测定的可行性。方法:按处方比例称取各中药,加入适宜的酶预处理,再按SBE法,采用正交实验L18(6×3^6)表,于第一煎和第三煎的溶剂中加入适宜的消化酶,在适宜的温度下提取得提取液。以该提取液为研究对象,HPLC法测定甘草次酸的含量,色谱条件:DiamonsilTM C18柱(5μm×4.6mm×250mm),流动相为甲醇:0.1%磷酸(87:13),流速为1ml/min,柱温为25℃,检测波长为250nm。结果:甘草次酸在0.145-1.740μg范围内线性关系良好,r=0.9999,平均加样回收率为98.14%,RSD=1.56%(11=6)。结论:半夏白术天麻汤的半仿生-酶法提取液可用HPLC测定其甘草次酸的含量,该方法准确、重现性好。
Objective:To optimize the craft of Banxia Baizhu Tianma Decoction extracted by Semi-bionic enzyme extraction, taking enoxolone as synthetic evaluation index to investigate the feasibility by HPLC.Methods: The traditional Chinese medicines were taken according to the prescription,pretreated by suitable enzyme,the L18 (6 ×3^6)table was used according to SBE method,suitable enzyme was added in the 1^st and 3^td decoction, then the extraction was obtained under suitable temperature.The content of enoxolone in this extraction was detected by HPLC with the following chromatographic condition:DiamonsilTM C18 column (5 μm × 4.6mm × 250mm), mobile phase:methanol-0.1% phosphonic acid (87:13),flow rate: 1 ml/min,column temperature:25℃,detecting wave length:250nm.Results:The linear rang of enoxolone was 0.145-1.740 μg,r=0.9999.The average recovery was 98.14%,RSD=1.56%(n=6),Conclusions:The content of enoxolone in Banxia Baizhu Tianma Decoction extracted by Semi-bionic enzyme extraction can be detected by HPLC.The result is accurate and the reproduction quality is well.
出处
《承德医学院学报》
2008年第2期119-120,共2页
Journal of Chengde Medical University