半仿生-酶法提取的半夏白术天麻汤中甘草次酸的含量测定

ASSAYING OF ENOXOLONE IN BANXIA BAIZHU TIANMA DECOCTION EXTRACTED BY SEMI-BIONIC ENZYME EXTRACTION

目的：为优选半夏白术天麻汤用半仿生-酶法提取的工艺条件，以甘草次酸为综合评判指标之一，探讨采用HPLC法测定的可行性。方法：按处方比例称取各中药，加入适宜的酶预处理，再按SBE法，采用正交实验L18（6×3^6）表，于第一煎和第三煎的溶剂中加入适宜的消化酶，在适宜的温度下提取得提取液。以该提取液为研究对象，HPLC法测定甘草次酸的含量，色谱条件：DiamonsilTM C18柱（5μm×4．6mm×250mm），流动相为甲醇：0．1％磷酸（87：13），流速为1ml／min，柱温为25℃，检测波长为250nm。结果：甘草次酸在0．145-1．740μg范围内线性关系良好，r=0．9999，平均加样回收率为98．14％，RSD=1．56％（11=6）。结论：半夏白术天麻汤的半仿生-酶法提取液可用HPLC测定其甘草次酸的含量，该方法准确、重现性好。

Objective：To optimize the craft of Banxia Baizhu Tianma Decoction extracted by Semi-bionic enzyme extraction, taking enoxolone as synthetic evaluation index to investigate the feasibility by HPLC.Methods： The traditional Chinese medicines were taken according to the prescription,pretreated by suitable enzyme,the L18 （6 ×3^6）table was used according to SBE method,suitable enzyme was added in the 1^st and 3^td decoction, then the extraction was obtained under suitable temperature.The content of enoxolone in this extraction was detected by HPLC with the following chromatographic condition：DiamonsilTM C18 column （5 μm × 4.6mm × 250mm）, mobile phase：methanol-0.1% phosphonic acid （87：13）,flow rate： 1 ml/min,column temperature：25℃,detecting wave length：250nm.Results：The linear rang of enoxolone was 0.145-1.740 μg,r=0.9999.The average recovery was 98.14%,RSD=1.56%（n=6）,Conclusions：The content of enoxolone in Banxia Baizhu Tianma Decoction extracted by Semi-bionic enzyme extraction can be detected by HPLC.The result is accurate and the reproduction quality is well.