摘要
目的:研究建立蟾酥HPLC指纹图谱分析方法,为其质量控制和药材鉴别提供依据。方法:利用高效液相色谱法,采用反相Alltima C18柱(250 mm×4.6mm,5μm);流动相:0.5%磷酸二氢钾溶液(磷酸调节pH为3.0)和乙腈(梯度洗脱);检测波长:296 nm;柱温:30℃;流速:1.0 mL/min。结果:在选定的色谱条件下确定10个峰构成蟾酥药材的指纹特征,各批次药材均具有上述特征,但特征峰的相对含量分布差异导致色谱概貌存在一定差异。确定了蟾酥HPLC中酯蟾毒配基(Resibufogenin)、华蟾酥毒基(Cinobufagin)、蟾毒灵(Bufain)、去乙酰基蟾毒它灵(Bufotalin)以及华蟾毒精(Cinobufotalin)所对应的保留时间一致;10个批次指纹图谱相似度均大于0.93。结论:该方法准确、简单、稳定,其色谱指纹图谱可用于蟾酥的鉴别和质量控制。
AIM： To establish HPLC fingerprint of Venenum Bufonis in an attempt to become a standard of quality control. METHODS ： The HPLC method was set up using Alhima C 18 （250 mm × 4.6 mm, 5 μm） column with mobile phase of acetonitrile-0.5 % potassium dihydrogen phosphate solution of water ; UV detection wavelength at 296 nm and column temperature at 30 ℃ with the flow rate of 1.0 mL/min ; 20 μL of the injection volume. RE- SULTS： In this chromatogram condition, 10 peaks were identified as the characteristic fingerprints of Venenum Bufonis. All samples showed the content differences among the samples. The retention times for resibufogenin ,cinobufagin,bufalin,bufotalin and cinobufotalin in Venenum Bufonis were consistent with each other. The fingerprint showed good similarity up to 93% in samples from different habitats. CONCLUSION： The method is exact ,simple and accurate, and can be used for the identification and quality control of Venenum Bufonis.
出处
《中成药》
CAS
CSCD
北大核心
2008年第5期625-628,共4页
Chinese Traditional Patent Medicine
基金
江苏省公益研究项目BM2004525
