薄荷油对大鼠肝组织GSH、ATP酶和原代肝细胞的影响

Effect of peppermint oil on GSH and ATPase in rat liver-tissues and primary cultured rat hepatocyte

目的:研究薄荷油对大鼠肝组织GSH、ATP酶和原代肝细胞的影响。方法:大鼠口服24%薄荷油,36 h和48 h后取肝组织,检测GSH含量以及Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力。分离培养原代大鼠肝细胞,加入薄荷油或其含药血清共同孵育,分别于12 h、24 h、48 h时检测上清液中LDH、ALT、AST水平。结果:肝组织GSH含量显著降低,Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力明显降低(P<0.05或P<0.01)。薄荷油0.5μL/mL、5μL/mL和10%薄荷油含药血清均可导致肝细胞上清液中LDH、ALT、AST水平不同程度的升高(P<0.05或P<0.01),在24 h最为显著,与四氯化碳引起的损害相似。结论:薄荷油口服能被机体吸收并直接对肝细胞产生损伤,其损伤途径可能与氧化应激反应有关。

To study the effect of peppermint oil on GSH ,ATPase in rat liver-tissues and primary cultured rat hepatocyte. METHODS ： The rat liver tissues were obtained to detect content of GSH and level of Na^2＋ -K^2＋ -AT- Pase ,Ca^2＋ -Mg^2＋ -ATPase after being administered peppermint oil orally at 24 % concentration of peppermint oil in 36 and 48 hours. Primary rat hepatocyte was separated and cultured, then peppermint oil and the serum containing that were respectively added level of LDH, ALT, AST in the supernatant fluid was respectively determined after in- cubating for 12, 24 and 48 hours. RESULTS： The content of GSH and level of Na^＋ -K^＋ -ATPase and Ca^2＋-Mg^2＋- ATPase in liver tissues was low remarkably （P 〈0.05 or P 〈0.01 ）. The level of LDH, ALT, AST in the supernatant fluid was high in 0.5 μL/mL peppermint oil group, 5 μL/mL peppermint oil group and 10% serum containing peppermint group（P 〈0.05 or P 〈0.01 ）, especially significant in 24 hours and similar to injury by carbon tetra- chloride group. CONCLUSION： Peppermint oil might be absorbed by liver and injury hepatocyte directly after being adminstered orally,in probable relation to oxidative stress reaction.