期刊文献+

RNA研究相关技术及其应用 被引量:1

RNA research - related techniques and their application
下载PDF
导出
摘要 RNA技术可以分为RNA基础研究相关的技术、RNA应用相关的技术和RNA的生物信息学技术。RNA基础研究相关技术包括RNA分离纯化和鉴定技术、RNA与其他生物大分子相互作用技术、RNA高级结构的研究技术和其他相关RNA技术;RNA应用相关技术则包括用于生产其他产品的RNA技术和直接用于药物开发的RNA技术;RNA的生物信息学技术则有各种数据库、非编码RNA的预测、RNA二级结构预测和各种设计软件。本文简略介绍了上述各类RNA技术的原理及其国内外研究进展,从而有助于对RNA领域有关技术方面有一较全面的了解。 RNA research- related techniques can be roughly divided into three types:techniques for basic research including techniques for RNA isolating/purifing and characterizing, study of interaction between RNA and other biomolecules and RNA structure analysis; techniques for applied research including techniques to make other products or directly develop diagnostic or therapeutic drugs; RNA bioinformatics techniques including various types of database, techniques for RNA prediction, RNA structure prediction and some designing tools. Here we briefly reviewed the principles and recent advances of RNA research - related techniques. It is hoped to give some overall introduction for the tools of RNA realm.
出处 《生命科学》 CSCD 2008年第2期183-189,共7页 Chinese Bulletin of Life Sciences
基金 "973"项目(2005CB724600)
关键词 RNA基础研究相关技术 RNA应用技术 RNA生物信息学技术 RNA techniques for basic research RNA techniques for applied research RNA bioinformatics
  • 相关文献

参考文献67

  • 1萨姆布鲁克J,拉塞尔D W,黄培堂,王嘉玺,朱厚础,等译.分子克隆实验指南[M]3版.北京:科学出版社,2002..
  • 2Feldman AJ, Costouros NG, Wang E, et al. Advantages of mRNA amplification for microarray analysis. Biotechniques, 2002, 33 (4): 906-14
  • 3Lagos-Quintana M, Rauhut R, Lendeckel W, et al. Identification of novel genes coding for small expressed RNAs. Science, 2001, 294(5543):853-8
  • 4Lau NC, Lira LP, Weinstein EG, et al. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science, 2001, 294(5543): 858-62
  • 5Lee RC, Ambros V. An extensive class of small RNAs in Caenorhabditis elegans. Science, 2001, 294(5543): 862-4
  • 6Ambion公司.PureLinkTM miRNA分离试剂盒说明书[Z].
  • 7Illumina公司.DASL Assay for RNA Profiling with Paraffin-Embedded Samples[Z].
  • 8Yeakley JM, Fan JB, Doucet D, et al. Profiling alternative splicing on fiber-optic arrays. Nat Bioteclmol, 2002, 20(4): 353-8
  • 9Haller AC, Kanakapalli D, Walter R, et al. Transcriptional profiling of degraded RNA in cryopreserved and fixed tissue samples obtained at autopsy. BMC Clin Pathol, 2006, 6:9
  • 10Bennett S. Solexa Ltd. Pharmacogenomics, 2004, 5(4): 433-8

二级参考文献34

  • 1Tuerk C,Gold L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase.Science,1990,249 (4968):505~510
  • 2Ellington A D,Szostak J W.In vitro selection of RNA molecules that bind specific ligands.Nature,1990,346 (6287):818~822
  • 3Cox J C,Ellington A D.Automated selection of anti-protein aptamers.Bioorg Med Chem,2001,9 (10):2525~2531
  • 4Cox J C,Hayhurst A,Hesselberth J,et al.Automated selection of aptamers against protein targets translated in vitro:from gene to aptamer.Nucleic Acids Res,2002,30 (20):e108
  • 5Stoltenburg R,Reinemann C,Strehlita B.FluMag-SELEX as an advantageous method for DNA aptamer selection.Anal Bioanal Chem,2005,383 (1):83~91
  • 6Wang C,Zhang M,Yang G,et al.Single-stranded DNA aptamers that bind differentiated but not parental cells:subtractive systematic evolution of ligands by exponential enrichment.J Biotechnol,2003,102 (1):15~22
  • 7Mendonsa S D,Bowser M T.In vitro evolution of functional DNA using capillary electrophoresis.J Am Chem Soc,2004,126 (1):20~21
  • 8Drabovich A,Berezovshi M,Krylov S N.Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM).J Am Chem Soc,2005,127 (32):11224 ~11225
  • 9Vater A,Jarosch F,Buchner K,et al.Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach:tailored-SELEX.Nucleic Acids Res,2003,31 (21):e130
  • 10Wen J D,Gray D M.Selection of genomic sequences that bind tightly to Ff gene 5 protein:primer-free genomic SELEX.Nucleic Acids Res,2004,32 (22):e182

共引文献237

同被引文献14

  • 1SUN Y, YE S H, LU H W. Study of RNA secondary struc-ture prediction algorithms [J]. Advanced Materials Research,2012, 393-395: 955-960.
  • 2LIU N, WANG T M. A method for rapid similarity analysisof RNA secondary structures[J/OL]. BMC Bioinformatics,2006,7 : 493. [2012-12-04]. http://www. biomedcentral.com/1471-2105/7/493.
  • 3HAVGAARD J,KAUR S, GORODKIN J. ComparativencRNA gene and structure prediction using Foldalign andFoldalignM [J]. Curr Protoc Bioinformatics, 2012, 2012(12): 12. 11. 1-12. 11. 15.
  • 4MATHEWS D H, TURNER D H. Dynalign: an algorithmfor finding the secondary structure common to two RNA se-quences [J]. J Mol Biol,2002,317(2) : 191-203.
  • 5ZUKER M. Calculating nucleic acid secondary structure [J].Curr Opin Struct Biol,2000, 10(3) : 303-310.
  • 6MATHEWS D H. Predicting a set of minimal free energyRNA secondary structures common to two sequences [J],Bioinformatics, 2005. 21(10): 2246-2253.
  • 7MOULTON V,ZUKER M, STEEL M, et al. Metrics onRNA secondary structures [J], J Comput Biol,2000,7(1-2) : 277-292.
  • 8WATTS J M, DANGKK,GORELICKRJ,et al. Architec-ture and secondary structure of an entire HIV-1 RNA genome[J]. Nature, 2009, 460(7256): 711-716.
  • 9KACHOURI R, STRIBINSKIS V,ZHU Y, et al. A surpris-ingly large RNase P RNA in Candida glabrata [J]. RNA,2005,11(7): 1064-1072.
  • 10TABEI Y, TSUDA K,KIN T, et al. SCARNA: fast and ac-curate structural alignment of RNA sequences by matchingfixed-length stem fragments [J]. Bioinformatics, 2006,22(14): 1723-1729.

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部