禾谷镰孢转化体系的优化

Optimization of Transformation System of Fusarium graminearum

禾谷镰孢是小麦赤霉病和玉米茎腐病的主要病原真菌。试验优化了聚乙二醇介导的原生质体转化法,建立了禾谷镰孢高效转化的稳定体系,并总结了影响转化成功的主要因素。以此方法对禾谷镰孢进行活体荧光标记,并用转化菌株侵染小麦胚芽鞘,在荧光显微镜下追踪病原真菌在宿主植物体内的生长及扩展过程。

Fusarium graminearum is one of the main pathogens of wheat head blight and maize stalk rot. In this paper, we optimized the transformation method using protoplasts mediated by polyethylene glycol （PEG）, therefore set up an efficient transformation system for E graminearum. We also discussed the key factors that affect the transformation efficiency. Using this method, we were able to transform F. graminearum with fluorescence marker containing plasmids. Inoculating wheat coleoptiles with fluorescence-labeled F. graminearum strain allows live tracking of the growth and spread of F. graminearum within host plants.