蓝光对拟南芥种子萌发的影响

The Effect of Blue Light on Seed Germination of Arabidopsis thaliana

通过检测分析不同蓝光条件下,拟南芥野生型和蓝光受体突变体的种子萌发率发现,蓝光诱导拟南芥种子萌发,隐花素主要介导蓝光诱导拟南芥种子的早期(蓝光培养1～3 d)萌发,并且与光照强度有关。施用GA生物合成抑制剂多效唑或嘧啶醇的研究结果表明,相同浓度的抑制剂对cry1cry2突变体种子萌发的抑制作用比野生型强,并且解除抑制剂对cry1cry2突变体种子萌发所需的外源有生物活性的GA_3量也较野生型多。这些实验结果初步证实了隐花素介导蓝光诱导种子萌发,并且可能与蓝光下种子萌发过程中有生物活性的GA合成增加有关。

Seed germination of wild type （WT） and blue light receptor mutant of Arabidopsis thaliana under blue light was analyzed, our results indicated that Arabidopsis seed germination was induced by blue light, and Cryptochrome mediated blue light induction of early seed germination （seeds were grown under blue light for less than three days）, and fluence rate dependent. Response of WT, cry1 and crylcry2 mutant seed germination to GA inhibitor pacolobutrazol and ancymidol was investigated, and crylcry2 mutant was more sensitive to GA inhibitor, and more GA3 was needed to recover inhibition of crylcry2 by GA inhibitor. We concluded that cryptochrome-mediated increase of GAs synthesis might responsible for cryptochrome-dependent induction of seed germination in response to blue light.