不同分化时期牛脂肪细胞抵抗素mRNA丰度变化

Abundance of resistin mRNA of calf adipocyte in different differentiation stage

选取犊牛大网膜脂肪组织,进行牛前脂肪细胞的单层贴壁培养,在细胞接种4、6、8、10、12、14、16d分别提取细胞总RNA,进行RT-PCR反应,以β-actin为定量内参照,对抵抗素mRNA的丰度进行定量分析,观察在犊牛前脂肪细胞向脂肪细胞分化过程中抵抗素mRNA的表达规律。结果表明:在犊牛前脂肪细胞分化4～12d,抵抗素mRNA丰度逐渐降低,而分化成熟后14～16d有所回升。

Preadipocytes from epiploon of calf were cultured with monolayer adherent method,and total RNA of adipocytes was extracted at 4,6,8,10, 12, 14 and 16 d, respectively. Abundance of resistin mRNA was analysed with intra-reference β-actin by relatively quantitative RT-PCR to observe the expression of resisfin mRNA during differentiation from preadipocyte to adipocyte. The result indicated that the abundance of resistin mRNA was down-regulated at 4-12 d of starting differentiation from preadipocyte to adipocyte,but up-regulated slightly 14-16 d after maturation.