场放大样品堆积毛细管区带电泳检测尿液中苯丙胺类毒品

Determination of amphetamines in urine by capillary zone electrophoresis with field-amplified sample stacking

建立了毛细管区带电泳（CZE）中场放大样品堆积（FASS）技术分析尿液中苯丙胺类毒品的方法。采用体积分数30%甲醇的100 mmol/L磷酸盐（pH 3）为分离缓冲液,利用缓冲体系与样品溶液体系电导率的差异,在毛细管中浓缩样品组分,对苯丙胺、甲基苯丙胺、3,4-（亚甲二氧基）苯丙胺（MDA）、3,4-（亚甲二氧基）甲基苯丙胺（MDMA）4种毒品进行了分离和定量测定,与常规毛细管区带电泳比较,检测灵敏度提高约2000倍。采用利多卡因为内标,对添加上述4种毒品的尿液进行提取和测定,分析的相对标准偏差在15%范围之内,可检测到的上述毒品质量浓度为0.002μg/mL,相对回收率在70%～120%内。该方法可用于生物检材中苯丙胺类毒品的检测。

An on-line concentration method of field-amplified sample stacking （FASS） by capillary zone electropheresis （CZE） was applied to analysising amphetamines in urine. With the buffer solution containing 100 nmol/L phosphate and 30% methanol （pH 3）, and the sample solution prepared by pure water, on-line concentration of 4 amphetamines： amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine （MDA）, 3,4-methylenemethamphtamine （MDMA） was accomplished in terms of the difference of conductivity between the buffer and sample solutions. Compared with the usual method of capillary zone electropheresis, the detection sensitivity increased up to about 2000-fold. The method was used to spiked urine with the relative standard deviation （RSD） of about 15%, the determination limit of 0.002 μg/mL and the recoveries of 70% - 120%, which promised the perspective application in detecting trace amount of amphetamines in hmnan body fluid.