水-乙醇溶液中肌红蛋白在琼脂糖膜中的氧化还原和电催化

Redox and electrocatalysis of myoglobin at agarose hydrogel films in aqueous/ethanol solution

用琼脂糖(Agarose)将肌红蛋白(Mb)固定在玻碳电极(GCE)表面,制备了Mb-Agrose膜修饰电极。在乙醇亲水性有机溶剂与水的混合溶液中,包埋在Agrose中的Mb可以与电极发生直接电子传递。紫外可见光谱表明水-乙醇溶液中琼脂糖膜中的肌红蛋白的构象保持不变。Mb-Agrose膜能快速地催化氯代乙烷(六氯乙烷、五氯乙烷和四氯乙烷等)脱氯,具有较好的稳定性和重现性,可用于这些物质的定量检测。

Myoglobin （Mb） was immobilized on a glassy carbon electrode （GCE） surface by agrose films. Mb entrapped in the agrose fihns underwent fast direct transfer-electron reactions in water-ethanol mixture. UV-Vis absorption （UV-Vis） spectra suggested that the Myoglobin keep their original conformation in the agarose fihns in water/ethanol mixture. The immobilized Mb is able to dehalogenate chloroethanes （hexachloroethane, pentachloroet hane, tetrachloroet hane）. Stability and reproducibility of the modified electrode could be used to determine the organic chloroethanes