固相微萃取-气相色谱/质谱法建立烟用香精指纹图谱

Establishment of fingerprint of tobacco flavor by SPME-GC/MS

采用固相微萃取法(SPME)对6批次的烟用HMT-4香精成分进行提取,GC/MS对其进行分析,建立了该香精的GC/MS指纹图谱的研究方法。通过参兑实验考察了色谱指纹图谱用于香精质量控制的可行性。结果表明:SPME法具有操作简便、快速、节能、萃取效率高等特点,适合于烟用香精成分的提取。6批次的HMT-4香精的指纹图谱稳定性好,相似度高,具有很强的特征性和唯一性。通过参兑实验发现色谱指纹图谱能全面、综合、准确地反映烟用香精的质量情况,可以成为烟用香精质量控制的有效手段。

The components of six batches of tobacco flavor HMT-4 samples were extracted with solid phase microextraction （SPME） and analyzed by GC/MS. The fingerprint of the flavor was established. By mixed test, the feasibility of chromatographic fingerprint in quality control of tobacco flavor was examined. The results showed that the SPME method has the characteristic of convenience, rapidity, less energy and high extraction efficiency, which is suitable for the extraction components of tobacco flavor. The fingerprint of the 6 batches of tobacco flavor HMT-4 samples have excellent stability, high similarity, great characteristic and tmiqueness. The chromatographic fingerprint can generally and exactly reflect the quality of tobacco flavor. The method can be used to control the quality of tobacco flavor.