儿茶素对小鼠造血祖细胞的刺激增殖作用

Effect of Catechin on the Proliferation of Hematopoietic Progenitor Cell in Mice

目的:观察儿茶素对小鼠造血祖细胞增殖作用。方法:通过造血祖细胞培养观察儿茶素对小鼠CFU-E、BFU-E、CFU- GM、CFU-Meg生长的作用。结果:加入儿茶素工作液后,CFU-GM、CFU-E、BFU-E、CFU-Meg集落数均明显升高。至100 mg·L^(-1)时,各系造血祖细胞集落数均增加至最高,与未加儿茶素的实验组相比均有统计学差异(P<0.01)。结论:儿茶素可明显促进小鼠造血祖细胞(CFU-GM、CFU-E、BFU-E、CFU-Meg)的增殖,并可与造血生长因子起协同效应,具有明显的造血促进作用。

Objective： To study the effect of eatechin on proliferation hematopoietie progenitor cell （HPC） in mice, Method： Cultivation of HPC in vivo was used to observe the influence of catechin on growth of CFU-E ,BFU-E ,CFU-GM ,CFU-Meg in mice. Result： The numbers of CFU-GM, CFU-E, BFU-E, CFU-Meg were obviously increased under the effect of eatechin. The numbers of CFU-GM, CFU-E, BFU-E, CFU-Meg reached to the maximum when 100 mg.ml^-l eateehin solution was added into the culture system, which were significantly higher than those of the group without catechin（ P 〈 0.01 ）. Conclusion： Catechin can obviously stimulate the proliferation of CFU-GM, CFU-E, BFU-E, CFU-Meg of mice.