卡托普利对胰岛素抵抗-高血压综合征大鼠TNF-α、NO和SOD水平的影响

The Effects of Captopril on Level of TNF-α,NO and SOD of Insulin Resistant-Hypertensive-Syndrome Rats

目的:研究卡托普利对胰岛素抵抗-高血压综合征大鼠动物模型的影响。方法:用高糖高盐高脂喂饲大鼠的方法建立胰岛素抵抗-高血压(IRH)病理模型,随机分为空白对照组、模型组和卡托普利组。正常对照组和模型对照组均肌注5%丙二醇,0.8 ml·kg^(-1)·d^(-1),qd×4周,卡托普利组肌注卡托普利7 mg·kg^(-1)·d^(-1),qd×4周,以上各组在给药后第2和第4周,各测血压一次,在第4周末,全部大鼠禁食过夜,末次给药2 h后,全部大鼠眼眶放血,离心分离血清,用酶联免疫分析仪测定肿瘤坏死因子(TNF-α),空腹血糖,一氧化氮(NO)、超氧化物歧化酶(SOD)含量及给药前后的血压变化。结果:给药4周后,卡托普利组大鼠的血压为(126±4.5)mmHg,2 h血糖(2 hBG)为(6.81±0.52)mmol·L^(-1),与模型对照组比较有明显降低(P<0.01)。同时,卡托普利能明显降低IRH大鼠的TNF-α含量(P<0.01),并明显降低胰岛素抵抗(IR)大鼠NO的含量,而使SOD水平有所上升。结论:卡托普利有效降低血糖值,有抗高血压,改善胰岛素抵抗作用。

Objective： To study the effect of captopril on insulin resistant-hypertensive of animal model of rats. Method： Feeding high fat-sugar-salt diets to set up the model of insulin resistant-hypertensive （IRH） rat. And the rats were divided into 3 groups. Blank control group and model control group intramuscular injection of 5% propanediol, 0.8 ml·kg^-1·d^-1, qd ,and 4 weeks together. The eaptopfil group intramuscular injection of eaptopfil 7 ml·kg^-1·d^-1, qd , and 4 weeks together, each group measure blood pressure in the 2nd and 4th week. At the 4th weekend, all rats were forbade food over night. Whole blood was collected 2 hours later after the last administration from the orbit, and serum was separated by eentrifugation. Measured the contents of tumor necrosis factor-a （TNF-α）, fasting blood glucose （FBG）, nitrous oxide （NO） and superoxide-dismutase （SOD） by enzyme immunoassay analyzer, compared the changes of blood pressure before and after medication. Result： 4 weeks after the administration, the blood pressure of the rats of eaptopill group is （ 126±4, 5 ） mmHg, 2 hours blood glueose（2hBG） is （6. 81 ±0, 52）mmol·L^-1 , compared with model control group were obviously lower（ P 〈 0.01 ）, In the meantime, eaptopril could significantly decrease the contents of TNF-α （P 〈 0. 01 ）, significantly decrease the NO, and the activity of serum SOD increase in IRH rats, Conclusion： The effects of eaptopfil can decrease blood pressure and enhance the sensitivity, decrease the contents of rennin in IRH rats.