HPLC法测定胃长泰口服液中黄芩苷的含量

Determination the Content of Baicalin for Weichangtai Oral Liquol by HPLC

目的:建立胃长泰口服液中黄芩苷的含量测定方法。方法:采用HPLC法,色谱柱Diamondsil C_(18)(150 mm×4.6 mm,5μm)。流动相为甲醇-水-磷酸(50:50:0.2)。检测波长278 nm。结果:黄芩苷进样量在0.21～1.05μg之间呈良好的线性关系(r=0.999 8)。平均回收率为99.03%,RSD为2.3%。结论:测定方法简便、准确,重复性好,可用于该产品的质量控制。

Objective： To establish a method for determination the content of baicalin in weichangtai oral liquor. Method： Diamondsil ClS colum （ 150 mm × 406 mm, 5 μm） was used. Mobile phase was methanol-water-phosphoric acid （ 50： 50： 0.2 ）. Detection wavelength was 278 nm. Result： Baicalin sample size located 0.21 - 1.05 μg, and favourable linear correlation （ r = 0. 999 8 ） was showed. Average recovery was 99.03% RSD was 2.3%. Conclusion： The method is convenient and accurate. Meanwhile, it also have good reproducibility, may be used to quality control of baicalin.