加味四逆散抗大鼠肝纤维化的实验研究

Effect of Jiaweisinisan on Liver Fibrosis

目的探讨加味四逆散抗大鼠肝纤维化的作用。方法采用二甲基亚硝胺(DMN)致肝纤维化的方法对健康雄性SD大鼠进行造模,以秋水仙碱为阳性对照组。经加味四逆散治疗4周后测血清丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)、总蛋白(TP)、白蛋白(ALB)、透明质酸(HA)及Ⅳ型胶原(C-IV)水平,并检测肝组织羟脯氨酸(HyP),同时观察肝组织的病理改变。结果加味四逆散治疗后,可显著降低血清ALT(P<0.01)、ALP(P<0.01)、GGT(P<0.05)、HA(P<0.01)、C-IV(P<0.05)及肝组织HyP(P<0.01),升高TP(P<0.05)及ALB(P<0.01)。肝组织病理切片表明,用药后病理改变明显减轻。结论加味四逆散对实验性大鼠有明显的抗肝纤维化作用。

Objective To explore the effect of Jiaweisinisan on liver fibrosis. Method The rat model of liver fibrosis was induced by Dimethylnirtosamine （DMN）, and treated with Jiaweisinisan for the experimental group and with colchicine tablets for the positive control group. After the four week treatment with Jiaweisinisan, the serum levels of ALT, ALP, GGT, TP, ALB, HA and C-Ⅳ were determined. Hydroxyproline（HyP） level in the liver was detected. Histopathology of the liver was also observed. Result After treatment with Jiaweisinisan, the serum levels of ALT, ALP, GGT, HA, C-Ⅳ and the HyP from hepatic tissue decreased significantly, while the levels of TP and ALB were increased. The pathological lesion of hepatic fibrosis was also significantly improved in post-treatment. Conclusion Jiaweisinisan has an obvious effect on liver fibrosis in the rat liver fibrosis model.