西茜铂对宫颈癌HeLa细胞体内外增殖抑制作用的研究

Antitumour effects of supramolecular platinum complex CCP on HeLa cell line vitro and in vivo

目的:评价超分子药物西茜铂(CCP)体内外抗宫颈癌HeLa细胞的作用。方法:细胞培养测定CCP对HeLa细胞的半数抑制浓度(IC50);建立HeLa细胞移植裸鼠模型,比较CCP与卡铂(CBP)的抑瘤作用;HE染色判断肿瘤组织的坏死,CD34抗体免疫组化染色观察肿瘤组织中微血管的生长状况。结果:CCP和CBP对HeLa细胞的IC50分别是(44.4±8.5)μg/L和(103.7±8.2)μg/L;在裸鼠移植瘤,高(20mg/kg)、中(15mg/kg)、低(10mg/kg)剂量CCP组和CBP组(20mg/kg)的抑瘤率分别为56.1%～56.6%、37.6%～50.8%、33.0%～41.7%和21.1%～39.2%;高、中剂量CCP组的抑瘤率明显高于CBP组(20mg/kg),瘤质量低于CBP组,差异有统计学意义,t值分别为2.98和2.52,P均≤0.05。与葡萄糖组对比,CCP组的肿瘤组织血管生成受到抑制,坏死明显。结论:CCP在体内外均具有抑制宫颈癌HeLa细胞的增殖作用,其作用明显高于CBP。

OBJECTIVE：To estimate the antitumour effects of supramolecular platinum complex CCP on HeLa cell line in vitro and vivo. METHODS： MTT method was used to examine the IC50 of CCP on HeLa cell line. Nude mice model was used to compare the antitumour effect of CCP and carboplatin in nude mice model of HeLa cell line. The tumour necrosis was shown by HE-stain and the angiogenesis was presented by the positive immunohistochemical dying of anti-CD34 antibody. RESULTS：The IC50 of CCP and carboplatin were （44.4±8.5） μmol/L and （103.7±8.2） μmol/L, respectively. In nude mice model, the inhibitory rates of high concentration （20 mg/kg）, middle concentration（15 mg/kg）, low concentration （10 mg/kg） of CCP and CBP （20 mg/kg） were 56.1%-56.6%, 37.6%-50.8%,33.0%-41.7% and 21.1%-39.2%. The inhibitory rates of high concentration（20 mg/kg） and middle concentration （15 mg/kg） of CCP were higher than that of Carboplatin （20 mg/kg）, and the tumor quantities were lower than those of carboplatin, which were statistically different by t value were 2.98 and 2.52, P≤0. 05. Compared to the glucose control group, it was more obvious in angiogenesis inhibition and necrosis in CCP group. CONCLUSION： Supramolecular drug CCP has the antitumour proliferation effects on HeLa cell line in vitro and vivo, of which are obviously higher than that of CBP.