SW480侧群细胞分选及其ABCG2的检测

Isolation of side population cells and detection of ABCG2 from SW480

目的:分选结肠癌细胞株SW480中的侧群细胞,检测ABCG2的表达情况,并探讨两者的关系。方法:用流式细胞仪分选SW480中的侧群细胞和非侧群细胞,并检测它们的细胞周期;用细胞免疫化学、激光共聚焦和流式细胞仪检测SW480中的ABCG2表达情况。结果:SW480中存在侧群细胞,比例为2.29%,其大部分细胞处于静止期,S、G2和M期细胞仅占16.14%,而非侧群细胞增殖期比例相对较高,S、G2和M期细胞占34.05%;SW480中存在ABCG2+细胞亚群,比例约为10%,处于低分化状态。结论:SW480中的侧群细胞具有相对静止的干细胞特性,ABCG2+细胞亚群具有低分化的特性。联合两种方法,分选癌侧群细胞中的ABCG2+细胞,将有可能进一步纯化癌干细胞,为今后分离癌干细胞提供实验基础。

OBJECTIVE： To investigate the relationship between side population cells and ABCG2^＋ subpopulation from SW480 cancer cell line. METHODS： Side population cells and non-side population cells were sorted by FACS from SW480, and these cell cycles were measured. ABCG2 expression of SW480 was detected by immunocytochemistry, immunofluorescence and FACS. RESULTS：The side population cells containing 2.29% existed in SW480. The proportions of S,G2 and M states were 16. 14% and 34. 05% in side population cells and non-population cells, respectively; ABCG2^＋ subpopulation containing 10 % of SW480 kept in poor differentiation. CONCLUSION： Side population cells keep in quiescence and ABCG2^＋ subpopulation in poor differentiation, and so isolation of ABCG2^＋ subpopulation from side population cells may be a useful method to purify cancer stem cells.