摘要
目的:分选结肠癌细胞株SW480中的侧群细胞,检测ABCG2的表达情况,并探讨两者的关系。方法:用流式细胞仪分选SW480中的侧群细胞和非侧群细胞,并检测它们的细胞周期;用细胞免疫化学、激光共聚焦和流式细胞仪检测SW480中的ABCG2表达情况。结果:SW480中存在侧群细胞,比例为2.29%,其大部分细胞处于静止期,S、G2和M期细胞仅占16.14%,而非侧群细胞增殖期比例相对较高,S、G2和M期细胞占34.05%;SW480中存在ABCG2+细胞亚群,比例约为10%,处于低分化状态。结论:SW480中的侧群细胞具有相对静止的干细胞特性,ABCG2+细胞亚群具有低分化的特性。联合两种方法,分选癌侧群细胞中的ABCG2+细胞,将有可能进一步纯化癌干细胞,为今后分离癌干细胞提供实验基础。
OBJECTIVE: To investigate the relationship between side population cells and ABCG2^+ subpopulation from SW480 cancer cell line. METHODS: Side population cells and non-side population cells were sorted by FACS from SW480, and these cell cycles were measured. ABCG2 expression of SW480 was detected by immunocytochemistry, immunofluorescence and FACS. RESULTS:The side population cells containing 2.29% existed in SW480. The proportions of S,G2 and M states were 16. 14% and 34. 05% in side population cells and non-population cells, respectively; ABCG2^+ subpopulation containing 10 % of SW480 kept in poor differentiation. CONCLUSION: Side population cells keep in quiescence and ABCG2^+ subpopulation in poor differentiation, and so isolation of ABCG2^+ subpopulation from side population cells may be a useful method to purify cancer stem cells.
出处
《中华肿瘤防治杂志》
CAS
2008年第10期737-740,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
结肠肿瘤
干细胞
P糖蛋白类
细胞
培养的
colonic neoplasms
stem cells
P-glycoproteins
cell, cultured