乳腺癌组织Cox-2和ER β mRNA表达及其相关性研究

Expressions of Cox-2 and ER β mRNA in human breast cancer and their relationship

目的:探讨乳腺癌组织中还氧化酶-2(Cox-2)与雌激素受体β(ERβ)mRNA的表达及其相关性。方法:以β-actin基因为内参照,应用逆转录聚合酶链反应(RT-PCR)技术,检测35例乳腺癌患者的乳腺癌组织、相应癌旁组织和相应正常乳腺组织中Cox-2和ERβmRNA表达,应用GeneTools软件对RT-PCR产物电泳带进行半定量分析。结果:35例样本中,癌组织Cox-2mRNA表达(29例)明显增高,全距为0.03～0.99,与相应癌旁组织表达(全距0.01～0.52)和正常乳腺组织表达(很弱或无表达)差异有统计学意义,P<0.05。Cox-2mRNA表达与年龄、原发肿瘤大小和TNM分期无关,P>0.05;而与淋巴结转移状况有关,P<0.05。癌组织中ERβmRNA表达水平(全距0～0.74)低于相应癌旁组织(全距0～0.90)和相应正常乳腺组织(全距0～1.14),P<0.05。ERβmRNA表达与年龄无关,P>0.05;而与原发肿瘤大小、TNM分期和淋巴结转移状况有关,P<0.05。Cox-2mRNA的表达与ERβmRNA表达无明显相关。结论:Cox-2和ERβ的mRNA表达与乳腺癌生物学特性有关,Cox-2与ERβmRNA表达无明显相关。

OBJECTIVE： To investigate cyclooxygenase-2 （Cox-2） and estrogen receptor β （ER β） expressions and evaluate their relationship in human breast cancer. METHODS： With β-action gene as reference, Cox-2 and ER β expressions were monitored in 35 samples of breast cancer tissues, paired adjacent breast tissues, and paired normal breast tissues by reverse transcription polymerase chain reaction （RT-PCR） and evaluated by a semi-quantitative method. RESULTS： The expression level of Cox-2 mRNA in breast tumor tissue was significantly upregulated （with a range of 0.03- 0. 99） compared with that in both paired adjacent breast tissues （with a range of 0.01-0. 52） and paired normal breast tissues （nearly no expression）, P〈0.05. The expression of Cox-2 was not correlated with the clinical parameters, such as age, primary tumor size, and TNM stage （P〉0.05）, but correlated with lymph node metastasis, P〈 0.05. A marked increase was detected in the expression level of ER β mRNA in breast carcinomas （with a range of 0-0.74） compared with that in the adjacent breast tissues （with a range of 0-0. 90） or normal breast tissues （with a range of 0-1. 14）, P〈0.05. The ER β expression level was not correlated with age （P〉0. 05）, but correlated with TNM stage, lymph node metastasis, and the histological grading, P 〈 0.05. CONCLUSION： Both Cox-2 and ER β expressions are significantly associated with the biological behavior of human breast cancer.