乳腺癌患者血清MK和VEGF表达及其临床意义的研究

Expressions and significance of MK and VEGF in serum of patients with breast cancer

目的:检测乳腺癌患者血清中中期因子(MK)与血管内皮细胞生长因子(VEGF)的表达,探讨MK和VEGE在乳腺癌中的关系及价值。方法:采用酶联免疫吸附法检测63例乳腺癌患者血清中MK和VEGE的表达情况,并与同期30例乳腺良性疾病和30例正常乳腺组织对照,分析其与浸润程度、TNM分期、淋巴结转移以及ER、PR表达状态等生物学行为的关系。结果:乳腺癌患者外周血中MK、VEGE的阳性表达率均高于对照组,差异有统计学意义,P<0.05。乳腺癌血清中MK、VEGF的表达与浸润程度、淋巴结转移情况、TNM分期明显相关(P<0.05),乳腺癌血清中VEGE表达与ER和PR有关,并与MK存在明显的相关性(r=0.343,P<0.01),MK表达与ER和PR无关,P>0.05。结论:MK和VEGE在乳腺癌的生长、发展中起到重要作用,并且两者存在一定的协同作用,共同检测可能对于乳腺癌的治疗和预测预后有重要价值。

OBJECTIVE： To detect the expressions of MK and VEGF in serum of patients with breast cancer, and evaluate the value and the relationship between MK and VEGF. METHODS： MK and VEGF were determined with enzyme-linked immunosorbent assays in 63 cases of breast cancer, 30 cases of benign breast diseases and 30 heathly controls, so as to analyze the relationships of them with tumor type, TNM stages, lymph nodes metastasis and the expressions of estrogen receptor and progestin receptor as well. RESULTS： The expression rates of MK and VEGF in serum of patients with breast cancer were higher than those of the controls,P〈0.05. There were significantly correlations of them with tumor type,TNM stages and lymph node metastasis,P〈0.05. The expression of VEGF in the serum of patients with breast cancer was correlated with ER and PR, and was significantly correlated with MK （ r = 0. 343, P 〈 0.01 ）, while MK was not correlated with ER and PR. CONCLUSION： MK and VEGF play important roles in the tumorigenesis and progression of breast cancer, and are correlated each other and have the high value by combined detection in the prognosis and treatment of breast cancer.