He-Ne激光在香菇速生高产菌株选育中的应用　Ⅱ酯酶同工酶分析及中试栽培

AN APPLICATION OF He-Ne LASER IN SELECTION OF FAST-GROWING AND HIGH-YIELDING LENTINUS EDODES STRAINS ⅡANALYSIS OF ESTERASE ISOZYME PATTERNS AND MID-SCALE CULTIVATION

采用He－Ne激光诱变选育的6株香菇菌种酯酶同工酶与亲本相比，酶带条纹数、迁移率等特征发生了明显的改变，表明了在激光作用下，香菇菌丝体遗传物质发生了变异．所选育的香菇新菌种中试栽培结果表明：新菌种在生产条件下，生长周期平均缩短20天以上，香菇产量最低平均提高10％以上，最高可提高22.5％．

The esterase isozyme patterns of the six Lentinus edodes strains which had been irradiated by He-Ne laser were much different from the starting strains in several important characteristics such as: the number of isozyme stripe, the moving rate. It proved that the heredity substance of Lentinus edodes had been changed in the irradiaction of laser.The mid-scale cultivation of the selected new strains indicacted that the growth cycle had been shortened by 20 days and the yield were increased by l0% on average and by 22. 5% at the most.