组织微阵列技术对胃癌组织p53和MDM2蛋白表达的同步检测与对应分析

Correspondence analysis and simultaneous detection of p53 and MDM2 protein expression in human gastric cancer using tissue microarray

目的:探讨胃癌组织中p53和MDM2蛋白表达的相互关系.方法:47例石蜡包埋胃癌组织制成组织阵列,应用免疫组织化学方法检测其p53和MDM2蛋白表达,并运用统计软件SPSS11.5对两个蛋白之间的相互关系进行对应分析.结果:p53与MDM2蛋白阳性表达率分别为34.0%(16/47)和42.5%(20/47).p53蛋白与MDM2蛋白表达呈负相关(P=0.012).结论:p53突变是胃癌发生发展中重要的一步,MDM2可能在胃癌的恶性增生过程中起重要作用.

AIM： To investigate the correlation between the expression of p53 and MDM2 proteins in human gastric cancer. METHODS： Tissue microarray and immunohistochemical method were used to detect the expression of p53 and MDM2 proteins in 47 patients with gastric cancer. The correlation between the two proteins was correspondingly analyzed using the SPSS11.5 software. RESULTS： The positive rates of p53 and MDM2 protein expression were 34.0% （16/47） and 42.5% （20/47）, respectively. MDM2 positive expression was predominant in the p53-negativesamples and MDM2 negative expression corresponded to p53 positive expression （P = 0.012）. CONCLUSION： p53 mutation is a significant step in gastric carcinogenesis, during which MDM2 protein may take an active part.