DLC-1基因甲基化检测与肝细胞癌复发转移的关系

Relationship between methylation status of DLC-1 gene and metastasis of hepatocellular carcinoma

目的:研究DLC-1基因甲基化检测与肝细胞癌(hepatocellular carcinoma,HCC)复发转移的关系.方法:73例HCC标本依据临床以及病理学特征被分为高侵袭组和低侵袭组;采用甲基化特异性PCR对不同侵袭组HCC之间DLC-1基因甲基化表达进行分析.结果:DLC-1甲基化表达率高侵袭组明显高于低侵袭组,二者之间有明显差异(χ2=4.3567P<0.05).DLC-1甲基化阳性与阴性患者之间AFP、HBV双阳性率有明显差异(χ2=4.4224P<0.05);TNM分期越后DLC-1甲基化程度越高(χ2=10.8478,P<0.05);短期随访发现DLC-1甲基化的HCC患者中位生存期低于非甲基化患者(9.45movs36mo,P<0.05).结论:DLC-1基因甲基化可作为HCC复发转移监测指标,并可作为靶向治疗HCC复发转移的新靶点.

AIM： To investigate the relationship between the methylation status of DLC-1 gene and metastasis of hepatocellular carcinoma （HCC）. METHODS： Seventy-three surgical specimens of human HCC were divided into high- and low-invasion groups according to their clinicopathological features. The methylation status of DLC-1 gene was detected in both groups by methylation-specific polymerase chain reaction （MSP）. RESULTS： The methylation level of DLC-1 gene in HCC specimens with high invasion was significantly higher than that in specimens with low invasion （χ^2 = 4.3567, P 〈 0. 05）. The doublepositive rate of alpha-fetoprotein （AFP） andhepatitis B virus （HBV） was significantly different between methylation-positive and -negative specimens （χ^2 = 4.4224, P 〈 0.05）. The methyla- tion status of DLC-1 gene was also related to TNM stages （χ^2 = 10.8478, P 〈 0.05）. After shortterm following-up, the median survival time was significantly different between HCC patients with positive- and negative methylation of DLC-1 gene （9.45 mo vs 36 mo, P 〈 0.05）. CONCLUSION： The aberrant methylation of DLC-1 gene may not only offer an effective method for the early auxiliary diagnosis of invasion and metastasis, but also serve as an new target for HCC therapies.