The influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes

Objective:To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse's oocytes.Methods:A total of 15 KunMing mice aged 10-14 weeks were undergone ovulation induction and oocytes collection.The oocytes were randomly divided into three groups.The first group was the control group and cultured without macrophage;the second group(experimental group 1)was co-cultured with macrophages right after oocytes collection;the third group(experimental group 2)was cultured alone after oocytes retrieval,and co-cultured with macrophages from 8 cell stage.Results:1.The fertility rate in control group(91.0%)and experimental group 2(93.7%)was significantly higher than that in experimental group 1(72.0%,P<0.001).2.Blastocyst formation rate of the fertilized oocytes in the experimental group 2(78.2%)was much higher than control group(60.3%,P<0.001).3.Blastocyst formation rate of the oocytes in experimental group 2(73.2%)was much higher than control group(54.8%)and experimental group 1(50.0%),which were statistically significant.Conclusions:1.Co-culture with peritoneal macrophages had an adverse influence on the fertilization process.2.Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.

Objective： To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse＇s oocytes. Methods： A total of 15 KunMing mice aged 10-14 weeks were undergone ovulation induction and oocytes collection. The oocytes were randomly divided into three groups. The first group was the control group and cultured without macrophage; the second group （experimental group 1） was co-cultured with macrophages right after oocytes collection; the third group （experimental group 2） was cultured alone after oocytes retrieval, and co-cultured with macrophages from 8 cell stage. Results：1. The fertility rate in control group （91.0%） and experimental group 2 （93.7%） was significantly higher than that in experimental group 1 （72.0%, P〈0. 001）. 2. Blastocyst formation rate of the fertilized oocytes in the experimental group 2 （78.20/oo） was much higher than control group （60.3%, P〈0.001）. 3. Blastocyst formation rate of the oocytes in experimental group 2 （73.2%） was much higher than control group （54.8%） and experimental group 1 （50.0%）, which were statistically significant. Conclusions： 1. Co-culture with peritoneal macrophages had an adverse influence on the fertilization process. 2. Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.