我国2型糖尿病人群脂联素基因多态性与冠心病的相关性研究

Adiponectin gene polymorphisms are associated with the risk of coronary artery disease in Chinese type 2 diabetic patients

目的研究我国2型糖尿病（T2DM）人群脂联素基因多态性是否与冠心病（CHD）风险的相关性。方法以190例T2DM患者为研究人群,其中159例伴有CHD;31人为对照组。应用PCR-RFLP技术对脂联素基因单核苷酸多态性（SNPs）-11365,-3964,＋45及＋276进行基因型和基因频率分析,并采用病例-对照方法,研究脂联素基因多态性与CHD的风险关系。结果（1）脂联素基因SNP＋45T等位基因携带者CHD的风险有增加趋势（OR=1.782,P〈0.05）。（2）脂联素基因SNP＋276G等位基因携带者CHD的风险显著增加（OR=1.866,95%CI1.069-3.258,P〈0.05）。（3）脂联素基因SNP＋45、＋276TG单体型携带者CHD的风险增加（OR=1.998,P〈0.05）;GT单体型对CHD具有保护性作用（R=0.386,95%CI0.173-0.863,P〈0.05）。（4）脂联素基因SNPs＋45、＋276TT/GG基因型组合携带者CHD的风险显著增加（校正OR=4.856,P〈0.05）。结论在我国2型糖尿病人群中,脂联素基因单核苷酸多态性＋45、＋276与冠心病的风险相关。

Objective To investigate the association of adiponectin gene polymorphisms with the risk of coronary artery disease in type 2 diabetic patients in China. Methods Of 190 patients with type 2 diabetes, 159 individuals with coronary artery disease （CHD group） and 31 controls （Non-CHD） were recruited in our study. The polymerase chain reaction-restriction fragment length polymorphism （ PCR- RFLP ） assay was adopted to genotype all the subjects for the single nucleotide polymorphisms （SNPs） in the adiponectin gene（ADIPOQ）. The frequencies of genotypes and alleles at the sites were compared in the CHD and control groups. Results （1） SNP ＋45T was associated with increased risk of coronary artery disease （CAD）in type 2 diabetics（OR= 1. 782,P=0. 049）. （2） SNP＋276G was associated with increased risk of CAD in type 2 diabetics（ OR=1. 866,P=0. 027 ）. （3） Haplotypes including SNPs ＋45 andS276 were shown to be associated with CAD risk in type 2 diabetic patients（P=0.03）. A haplotype of TG was associated with increased risk of CAD（OR= 1. 998, P = 0. 014）. A haplotype of GT played a protective role in the risk of CAD （OR=0. 386, P= 0. 017）. （5） After adjusted for the known cardiovascular risk factors,genotypic combination of SNPs ＋45 and ＋276 was associated with the risk of CAD in type 2 diabetic patients. TT/GG combination was associated with increased CAD risk in type 2 diabetic patients（OR =4. 856,P=0. 038）. Conclusions Adiponectin gene SNPs＋45 and ＋276 are associated with risks for coronary artery desease in Chinese type 2 diabetic patients.