喹胺醇诱导仔猪氧化损伤的潜在性作用

Potential induction of kuianchun to oxidative injury in weanling piglets

12头断奶仔猪随机分为2组,分别单剂量口服或静脉注射喹胺醇,检测了给药后48 h内血抗氧化酶活力、MDA含量和血药浓度,旨在评估喹胺醇诱导氧化损伤的潜在性作用。结果,猪单次口服200 mg/kg喹胺醇后,血CAT活力在2～48 h间显著高于给药前（P〈0.05）,GSH含量与CAT活力变化呈相反趋势（P〈0.05）,SOD、GSH-Px、GSH-ST活力、MDA含量未见明显变化;单次注射3.0 mg/kg喹胺醇后,SOD活力在5～30 min内显著低于给药前（P〈0.05）,MDA含量与之呈相反变化,CAT、GSH-Px、GSH-ST活力、GSH含量给药前后均无显著性差异;血药浓度与抗氧化酶活力均呈负相关性,与MDA含量呈正相关性。结果表明,断奶仔猪单剂量口服或注射喹胺醇后,有导致抗氧化机能短暂紊乱的可能,但不会诱发氧应激性损伤。

In order to investigate the potential induction of kuianchun to oxidative injury,12 weanling piglets were divided into 2 groups randomly,and administered kuianchun orally at high single dose and injected kuianchun in vein at little single dose,respectively.The activity of antioxidant enzyme and concentration of MDA in blood were determined spectrophotometrically,drug concentration in plasma was measured by RP-HPLC.It was found that the activity of CAT in blood increased significantly（P〈0.05）,concentration of GSH decreased significantly（P〈0.05） from 2 hours after orally administered kuianchun,but the activity of SOD,GSH-Px,GSH-ST or MDA concentration were not significant changes.After injected kuianchun in vein,SOD activity decreased significantly from 5 to 30 minutes,MDA changes was opposite result to SOD.However,all these changes were not observed after injected drugs for 8 hours.CAT,GSH-Px,GSH-ST,and GSH were not affected significantly by injected drugs.A negative correlation between drug concentration of plasm and antioxidant enzyme activity,and a positive correlation between drug concentration and MDA concentration were observed.These results suggested that oxidative injury was not induced by being administered kuianchun at single dose,but the disorder of antioxidative property was observed.