5种弥散神经内分泌细胞在鸭腔上囊中的表达特征(英文)

Expression patterns of five kinds of diffuse neuroendocrine system (DNES) cells in duck bursa of Fabricius

应用免疫组化技术并结合图像分析软件研究了胃泌素、β-内啡肽、胰高血糖素、5-羟色胺、生长抑素在鸭腔上囊中的表达特征。结果显示:鸭腔上囊小结相关上皮细胞呈胃泌素强阳性反应;滤泡间粘膜上皮呈β-内啡肽中强度阳性反应;淋巴滤泡皮质部有少量的β-内啡肽、胃泌素、5-羟色胺阳性细胞,呈中、强度免疫反应;淋巴滤泡髓质有胃泌素强阳性细胞;生长抑素和胰高血糖素均呈阴性反应。结果说明,鸭腔上囊中存在β-内啡肽、胃泌素、5-羟色胺弥散神经内分泌细胞,它们在淋巴滤泡皮质部的表达特征有利于其通过内分泌和旁分泌方式调节B细胞的发育。

The expression patterns of gastrin（Gas）,β-endorphin（End）,somatostatin（SS）,glucagons（Glu）,and 5-hydroxytryptamine （5-HT） cells inthe duck bursa of Fabricius were studied by the immunohistochemical method associated with image analysis.The results showedthat the Gas-positive cells with high intensity were seen in the follicle-associated epithelium（FAE）,the End with medium intensity in the interfollicular epithelium,the End,Gas and 5-HT with medium or high intensity in the cortex of lymphoid follicles,and the Gas with high intensity in the medulla of lymphoid follicles.Both SS and Glu showed negative reaction.The present results demonstrated that the Gas,End and 5-HT cells are differentially expressedin the different parts of the duck bursa.The positive cells distributed in the cortex of lymphoid follicles might favour exerting their influence via an endocrine,autocrine or paracrine path ways on the development of B cells.