RT-PCR快速检测牛病毒性腹泻病毒方法的建立与应用

Development of RT-PCR assay for rapidly detecting of bovine viral diarrhea virus

根据已发表的牛病毒性腹泻病毒(BVDV)5'-UTR序列,设计1对特异性引物,建立了快速检测BVDV的RT-PCR方法。利用该方法能从BVDV中扩增出196bp特异性目的片段。应用此方法检测了26份疑似病例临床样品,14份为阳性。试验表明,该方法特异性强、敏感性好,能检测到1pg的病毒RNA,是一种快速准确的检测方法。

According to the published sequences of the BVDV strains, a pairs of specific primers was designed, and then the RT-PCR method was established for detecting the bovine viral diarrhea virus. Using the specific primers 196 bp bands could be obtained from the BVDV by this method. Clinical samples were detected using the RT-PCR method, and 14 out of 23 samples were positive. The results indicated that the RT-PCR is very specific and sensitive,and could detect 1 pg virus＇ RNA.So it would be a quick and exact method for detecting the BVDV.