期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

吉戎兔微卫星DNA 被引量：1

Genetic polymorphism identified by microsatellite markers in Jirong rabbit
下载PDF
导出
摘要 选用Sat2、Sat3、Sat4、Sat5、Sat7、Sat8、Sat12、Sat13、Sat16、Sol08、Sol28、Sol30、Sol03等13个微卫星位点PCR扩增60只吉戎兔的基因组DNA,然后在8%聚丙烯酰胺凝胶上电泳分型。结果显示,平均等位基因数为3.46个,平均杂合度(H)为0.578,平均多态信息含量(PIC)为0.531。结果表明,吉戎兔经多年选育,其品种的遗传结构稳定,品质均一,且群体保持了较高的遗传多样性。 Thirteen microsatellite markers which consist of Sat2, Sat3, Sat4, Sat5, Sat7, Sat8, Sat12, Sat13, Sat16, So108, So128, So130, So103 were used to identify the paternity of 30 Jirong rabbits. Primers for polymerase chain reaction （PCR） were synthesized and 13 microsatellite loci were successfully amplified and the PCR products analysed by 8% lyacrylamide gel electrophoresis. The data were analysed with Cervus2.0 software. The results showed that the average number of the alleles in the 13 microsatellite loci was 3.46 b, the mean heterozygosity（ H ） and polymorphism information content （PIC） were 0.578 and 0.531, respectively, indicating that the genetic structure of Jirong rabbit population is stable, its genetic quality is uniform and there is a relatively high genetic polymorphism in Jirong rabbit.
作者 韩春梅 张嘉保 高庆华 李宏建
机构地区 吉林大学实验动物中心 塔里木大学动物科技学院 新疆生产建设兵团畜牧兽医总站
出处 《中国兽医学报》 CAS CSCD 北大核心 2008年第4期438-441,共4页 Chinese Journal of Veterinary Science
基金 国家自然科学基金资助项目(30170680)
关键词 吉戎兔 遗传多样性 微卫星DNA JIRONG rabbit polymorphism microsatellite marker
分类号 Q78 [生物学—分子生物学] S852.2 [农业科学—基础兽医学]
  • 相关文献

参考文献12

  • 1萨姆布鲁克 J,弗里奇 E F,曼尼阿蒂斯 T.分子克隆实验指南[M].金冬雁,黎孟枫,侯云德,等译.2版.北京:科学出版社,1999.
  • 2姜泊 张亚鹏 周殿元.分子生物学常用实验方法[M].北京：人民军医出版社,1997.128-130.
  • 3Mougel F,Mounolou J C,Monnerot M,et al.Nine polymorph-ic microsatellite loci in the rabbit(Oryctolagus Cuniculus)[J].Anim Genet,1997,28(1):58-59.
  • 4Rico C,Rico I,Webb N,et al.Four polymorphic microsatelli-te loci for the European rabbit (Oryctolagus Cuniculus)[J].Anim Genet,1994,25(5):367.
  • 5徐宁迎,傅衍,C.Looft,N.Reinsch,E.Kalm.多重聚合酶链反应筛选方法的研究[J].畜牧兽医学报,2001,32(2):108-112. 被引量：8
  • 6郑秀芬.法医DNA分析[M].北京:中国人民公安大学出版社,2001.
  • 7Vicente J S,Viudes-de-Castro M P,Lavara R,et al.Polymor-fismos de 13 microsatèlites en una linea seleccionada de conejos[J].Anim Genet,2000,11:33-36.
  • 8Guillaume Queney,Nuno Ferrand,Steven Weiss,et al.Station-ary distributions of microsatellite loci between divergent population groups of the European rabbit (Oryctolagus cuniculus)[J].Mol Biol Evol,2001,18:2169-2178.
  • 9Zenge K R,Vachot-Griffin A M,Richardson B J.A rapid population expemsion retains genetic diversity within Europeanrabbits in Australia[J].Mol Ecol,2003,12(3):789-794.
  • 10Surridge A K,Bell D J,Rico C,et al.Polymorphic micro satellite loci in the European rabbit (Oryctolagus cuniculus) are also amplified in other lagomorph species[J].Anim Genet,1997,28(4):302-305.

二级参考文献23

  • 1[1] Barendse W，Armitage S M，Kossarek L M，et al．A genetic linkage map of the bovine genome[J].Nature Genetics，1994，6（3）:227～235．
  • 2[2] Bishop M D，Kappes S M，Keele J W，et al．A genetic linkage map for cattle[J].Genetics，1994，136（2）:619～639．
  • 3[3] Eggen A，Fries R．An integrated cytogenetic and meiotic map of the bovine genome[J].Animal Genetics，1995，26（4）:215～236．
  • 4[4] Oetting W S，Lee H K，Flanders D J，et al．Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers[J].Genomics，1995，30，450～458．
  • 5[5] Heyen D W，Beever J E，Da Y，et al．Exclusion probabilities of 22 bovine microsatellite markers in fluorescent multiplexes for semiautomated parentage testing[J].Animal Genetics，1997，28，21～27．
  • 6[6] Rolfs A，Schuller I，Finckh U，et al．PCR:Clinical Diagnostics and Research[J].Springer Verlag Berlin Heidelberg，1992．
  • 7Vanhala T, Tuiskula-Haavisto M, Elo K, Vilkki J, Maki Tanila A. Evaluation of geuetic variability and genetic distance between eight chicken lines using microsatellite markers.Poultry Science, 1998, 77:783-790.
  • 8Zhou H, lamont S J. Genetic characterization of biodiversity in highly inbred chicken lines by microsatellite markers. Animal Genetics, 1999, 30:256-264.
  • 9Romanov M N, Weigend S. Analysis of genetic relationship between various populations of domestic and Jungle Fowl using microsatellite markers. Poultry Science, 2001, 80:1057 -1063.
  • 10Weigend S, Romanov M N. Current strategies for the assessment and evaluation of genetic diversity in chicken resources.World ' s Poultry Science Journal, 2001, 57:275 -288.

共引文献57

同被引文献18

  • 1朱玉峰,任文陟,张嘉保,王元占,郭雄明,周立波.家兔微卫星标记群体遗传变异快速分析方法的建立[J].中国比较医学杂志,2004,14(6):363-367. 被引量：12
  • 2陈民利,赵伟春,应华忠,王德军,徐孝平.WHBE兔遗传特异性的RAPD分析[J].浙江大学学报（农业与生命科学版）,2005,31(4):493-498. 被引量：23
  • 3李瑞生,董罡,吴晓燕,王鹏,王晓辉,陈振文.微卫星DNA监控大鼠近交系的培育[J].遗传,2006,28(7):821-824. 被引量：7
  • 4萨姆布鲁克J,拉塞尔D W,黄培堂,王嘉玺,朱厚础,等译.分子克隆实验指南[M]3版.北京:科学出版社,2002..
  • 5Chantry-Darmon C,Urien C,Hayes H,Bertaud M,Chadi-Taourit S,Chardon P,Vaiman D,Rogel-Gaillard C.2005.Construction of a cytogenetically anchored microsatellite map in rabbit [J].Mamm Genom,16(6):442-459.
  • 6Korstanje R,Gillissen GF,Versteeg SA,van Oost BA,Bosma AA,Rogel-Gaillard C,van Zutphen LF,van Lith HA.2003.Mapping of rabbit microsatellite markers using chromosome-specific libraries [J].J Hered,94 (2):161-169.
  • 7Mougel F,Mounolou JC,Monnerot M.1997.Nine polymorphic microsatellite loci in the rabbit,Oryctolagus cuniculus [J].Anim Genet,28:58-71.
  • 8Rico C,Rico I,Webb N,Smith S,Bell D,Hewitt G.1994.Four polymorphic microsatellite loci for the European wild rabbit,Oryctolagus cuniculus [J].Anim Genet,25(5):367.
  • 9Surridge AK,Bell DJ,Rico C,Hewitt GM.1997.Polymorphic microsatellite loci in the European rabbit (Oryctolagus cuniculus) are also amplified in other lagomorph species [J].Anim Genet,28(4):302-305.
  • 10谢建云,邵伟娟,胡建华,高诚.微卫星技术对近交系小鼠遗传质量的分析[J].中国比较医学杂志,2007,17(9):511-515. 被引量：7

引证文献1

二级引证文献5

中国兽医学报
2008年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部