水中甲胺磷的毛细管胶束电动色谱测定法

Determination of Methamidopho in Water by Micellar Electrokinetic Capillary Chromatography

目的探讨水中甲胺磷的毛细管电泳测定法。方法利用毛细管胶束电动色谱法对水中的甲胺磷进行测定,以保留时间定性,外标法定量。结果甲胺磷测定的最佳条件是:pH=7.5,20 mmol/LNaH2PO4-Na2HPO4缓冲液,30 mmol/LSDS,检测波长为254 nm,分离电压为25 kV,进样时间为9 s。该方法的线性范围为1.0～200μg/ml,r=0.999 1,回收率为91.5%～105.2%,RSD为1.35%～3.24%,检出限为0.007 1μg/ml(进样体积为3μl)。结论该方法灵敏,准确,能对甲胺磷残留进行快速检测。

Objective To develop a capillary electrophoresis method for the determination of methamidopho residue in water. Methods Methamidopho in water was determined by micellar electrokinetic capillary chromatograph. The qualitative analysis was done by retention time and quantitative analysis by external standard curve. Results The optimum conditions for methamidopho determination were： pH= 7.5, 20 mmol/L phosphate buffer, 30 mmoL/L SDS, wavelength of 254 nm, separate voltage 25 kV, inject duration of 9 s. The linear range was 1.0-200 μg/ml, the correlation coefficient was 0.999 1, the rates of recovery were 91.5%-105.2%, the relative standard deviations were 1.35%-3.24% for this method. The limit of determination was 0.007 1 μg/ml （capillary volume was 3 μl）. Conclusion This method was sensitive, accurate and applicable to rapid the determination of methamidopho.