76株淋病奈瑟菌药物敏感性试验及质粒检测

Antimicrobial Susceptibilities and Analysis of Plasmid 76 Strains of Neissria gonorrhoeae

目的为临床合理选择抗淋病奈瑟菌药物提供参考依据。方法采用改良Ｋａｄｏ法进行质粒鉴定；采用琼脂稀释法测定青霉素Ｇ等７种药物对７６株不同质粒类型的淋病奈瑟菌最低抑菌浓度（ＭＩＣ）。结果含２４．５×１０６质粒的４９株菌株中，３株含４．４×１０６Ｒ质粒，β－内酰胺酶阳性，并同时显示耐青霉素Ｇ（≥２μｇ／ｍｌ）、盐酸四环素（≥４μｇ／ｍｌ）和红霉素（≥２μｇ／ｍｌ），８株虽不含４．４×１０６质粒，也表现对青霉素Ｇ（≥１μｇ／ｍｌ）、盐酸四环素（≥２μｇ／ｍｌ）和氨苄西林（≥４μｇ／ｍｌ）的交叉耐药。与不含２４．５×１０６质粒组（０／２７）比较，差异有显著性（Ｐ＜０．０５）。未发现耐壮观霉素菌株。结论２４．５×１０６质粒与淋病奈瑟菌对青霉素Ｇ、盐酸四环素和氨苄西林的交叉耐药形成有关；

Objective To detect the minimun inhibitory concentrations of 7 antibiotics against 76 strains of Neisseria gonorrheae, as to investigating the changes of resistance of neisseria gonorrhoeae, providing physicians with the best selection of medicine. Methods Plasmid was extracted according to the method of Kado and Liu. The minimun inhibitory concentrations of each antibiotic was assessed using the agar plate dilution method. Results Plasmid profiles and antimicrobial susceptibilities of 76 strains of Nesseria gonorrhoeae were analyzed. A total of 49(64.5%) of 76 strains contained the 24.5×106a conjugative plasmid. Of these 49 strains, 3 PPNG strains contained the 4.4×106 R plasmid showed cross resistance between penicillin, (≥2 μg/ml) tetracyclineHce (≥4 μg/ml), and erythromycin (≥2 μg/ml). Eight strains showed cross resistance between penicillin, (≥1 μg/ml) tetracyclineHce (≥2 μg/ml), and ampicillin (≥4 μg/ml). Those strains contained the 24.5 plasmid were significantly (P<0.05) more than that the plasmid free (24.5×106) strains to penicillin, tetracyclineHce and ampicillin. Conclusions The results showed that the 24.5×106 conjugative plasmid has relation to cross resistance between penicillin, tetracycineHce and mpicillin. Spectinomycin is still proposed as the first candidate drug of Neisseria gonorrhoeae infection.