WOW培养法的研究

The Study of WOW Culturing Method

WOW培养法是目前培养胚胎的重要方法之一,在去透明带胚胎的培养上尤为重要。将拣卵针烧制成圆球状,在四孔板底烫制WOW,将不同时间去除透明带的牛孤雌激活胚培养于WOW中。结果显示:各组的卵裂率(78.9%、81.1%和78.9%)与对照组无显著差异(P>0.05);移入6-DMAP中之前去除组的囊胚率(31.5%)与移入培养液中之前去除组(32.4%)无显著差异(P>0.05),而且分别与对照组无差异显著(P>0.05);移入离子霉素中之前去除组未得到囊胚。移入6-DMAP中之前去除组囊胚细胞数(113.8±10.1)与移入培养液中之前去除组囊胚细胞数(112.5±8.1)和对照组均无显著差异(P>0.05)。分别5枚、15枚和30枚为一组进行培养,对胚胎的后续发育无显著影响。

WOW is one of the important methods for embryos culture, especially for zona-free embryos in vitro. In this study, glass needle was used to make WOW at the bottom of the four-well dish, and bovine zona-free parthenogenic activated embryos were cultured in the WOW. The results showed that cleavage rate of the three groups （78.9%, 81.1% and 78.9%） had no significant difference with control （P〉0.05）. The blastocyst rate of the group of zona being digested before putting in 6-DMAP （31.5%） had no significant difference with the group of zona being digested before putting in SOFaa （P〉0.05）, and the two groups had no significant difference with control （P〉0.05）. The group of zona being digested before putting in ionomycin didn’t develop to blastocyst. The number of cells had no significant difference between the group of zona being digested before putting in 6-DMAP and the group of zona being digested before putting in SOFaa, and have no significant difference with control （P〉 0.05）. Culturing embryos with different numbers in each group （5, 15, 30）, there is no different on the development of embryos.