高能聚焦超声治疗膀胱肿瘤的实验研究

An experimental study of the treatment of mice bladder carcinoma with high intensity focused ultrasound

为探索高能聚焦超声（HIFU）经腹治疗小鼠膀胱肿瘤的可行性，采用同系移植技术建立小鼠膀胱可移植性移行细胞癌模型，观察：①HIFU对膀胱荷瘤鼠生存期的影响；②荷瘤鼠生存曲线的变化；③HIFU对荷瘤鼠肿瘤体积及肿瘤重量的影响；④靶区肿瘤组织以及邻近组织超微结构变化。结果荷瘤鼠生存时间治疗组为22．90±4．78d，对照组为14．10±3．52d，两组有非常显著性差异（P＜0．01）；HIFU可导致肿瘤组织凝固性坏死，明显减轻肿瘤负荷。认为HIFU可以治疗小鼠膀胱肿瘤，为下一步临床研究奠定了理论基础。

To study the feasibility of the treatment of bladder carcinoma with high intensity focused ultrasound (HIFU) through a transabdominal approach in T_(739), mice,a model of orthotropic transplantation bladder transitional cell cancer was established by implanting BTT_(739). cells of bladder transitional cell cancer in syngenic mice. Using this model,Bladder carcinoma was treated with a HlFU device. The survival time(day),the survival curve change,mean tumor volume and weight,and microscopic pathology and ultrastructure of the treated zone were investigated. The results showed that mice survival time of treated group (22. 90± 4. 78 days) was significant longer than that of control group (14. 10±3. 52 days) (P <0. 01). HIFU can reduce the tumor weights greatly (P <0. 01). Histological observation showed direct thermal cytotoxic coagulative necrosis in the treated zone,and HIFU could decrease load of carcinoma signficantly. HIFU could be used focally to ablate mice orthotopic bladder carcinoma and the next step will be to determine whether this type of therapy can be adapted to the treatment of human bladder carcinoma.