Differential expression and regulation of integral membrane protein 2b in rat male reproductive tissues

Aim： To examine the expression and regulation of integral membrane protein 2b （Itm2b） in rat male reproductive tissues during sexual maturation and under different treatments by in situ hybridization. Methods： Testis, epididymis, and vas deferens were collected on days 1-70 to examine Itm2b expression during sexual maturation. To further examine the regulation of Itm2b, adult rats underwent surgical castration and cryptorchidism. Ethylene dimethane sulfonate and busulfan treatments were carried out to test the regulation of Itm2b after destruction of Leydig cells and germ cells. Results： In testis, Itm2b expression was moderately detected in the adluminal area of seminiferous cords on days 1-10, and detected at a low level in the spermatogonia on days 20 and 30. The Itm2b level was markedly increased in Leydig cells from day 20 to day 70. In epididymis and vas deferens, Itm2b was detected from neonate to adults, and the signal gradually increased in accordance with sexual maturation. Itm2b expression was significantly downregulated in epididymis and vas deferens of castrated rats, and strongly stimulated when castrated rats were treated with testosterone. Cryptorchidism led to a significant decline of Itm2b expression in testis and caput epididymis. Itm2b expression in epididymis and vas deferens was significantly decreased after the Leydig ceils were destroyed by ethylene dimethane sulfonate. Busulfan treatment produced no obvious change in Itm2b expression in epididymis or vas deferens. Conelusion： Our data suggested that Itm2b expression is upregulated by testosterone and might play a role in rat male reproduction.

瞄准：在老鼠男性检验不可分的膜蛋白质 2b (Itm2b ) 的表示和规定繁殖纸巾在性成熟期间并且在不同治疗由下面在 situ 杂交。方法：睾丸， epididymis，和管推迟 ens 在天 1-70 上被收集在性成熟期间检验 Itm2b 表示。为了推进，检验 Itm2b 的规定，成年老鼠经历了外科的阉割和 cryptorchidism。乙烯暗淡乙烷 sulfonate 和 busulfan 治疗被执行在 Leydig 房间和细菌房间的破坏以后测试 Itm2b 的规定。结果：在睾丸， Itm2b 表示中等在天 1-10 上在生精的绳索的广告钠区域被检测，并且在天在 spermatogonia 在底层检测了 20 和 30。Itm2b 水平显著地从到白天 70 的白天 20 在 Leydig 房间被增加。在 epididymis 和管推迟 ens， Itm2b 从出生不满一月的婴儿被检测到逐渐地根据性成熟增加的成年人，和信号。Itm2b 表示是显著地击倒在 epididymis 和管调整了推迟阉割的老鼠的 ens，并且当阉割的老鼠与睾丸激素被对待时，强烈刺激了。Cryptorchidism 在睾丸和头 epididymis 导致了 Itm2b 表示的重要衰落。在 epididymis 和管的 Itm2b 表示推迟在 Leydig 房间被乙烯破坏以后， ens 显著地被减少暗淡乙烷 sulfonate。Busulfan 处理没在 epididymis 或管在 Itm2b 表示生产明显的变化推迟 ens。结论：我们的数据建议那个 Itm2b 表达式起来在老鼠男性复制由睾丸激素和力量玩调整了一个角色。