抗变链c型表面蛋白P1抗体对变链粘附的影响

Effects of Different Dilution of Specific Antibodies Against the Cell Surface Protein P1 of S.mutans MT 6R upon its Adherence

用提纯的变形链球菌（血清型ｃ）细胞表面蛋白Ｐ１加弗氏佐剂对ＢＡＬＢ／Ｃ鼠行腮腺区和背部皮下注射免疫，得到含高效价抗Ｐ１抗体的鼠血清和唾液。将血清和唾液样本分别作梯度稀释，观察不同稀释倍数的抗Ｐ１抗体对Ｓ．ｍｕｔａｎｓＭＴ６Ｒ粘附的影响。结果发现原倍及稀释度为１∶２，１∶４，１∶８，１∶１６，１∶３２的抗变链Ｃ型表面蛋白Ｐ１血清抗体和唾液抗体均可明显抑制Ｓ．ｍｕｔａｎｓＭＴ６Ｒ在唾液包被的羟磷灰石微珠（Ｓ－ＨＡ）表面的粘附（Ｐ＜０．０５）。随着抗体稀释倍数的增加，抑制作用逐渐降低，稀释倍数达６４倍时，抗Ｐ１抗体对ＭＴ６Ｒ的粘附无明显抑制效应（Ｐ＞０．０５）。

The sera and saliva were obtained from BALB/C rats by injection of the cell surface protein P1 and Freud's adjuvant. High antibody titers were showed in the sera and saliva by an enzymelinked immunosorbent assay. Different dilution of the specific antibodies against the protein P1 of S.mutans had different inhibition effects on adhereing of S.mutans MT 6R. Adherence of streptococcus mutans MT 6R on salivacoated hydroxyapatite was reduced significantly by using sera and salivary antibodies in dilution of 1∶1, 1∶2, 1∶4, 1∶8, 1∶16, 1∶32 (P<0.05).