由簇毛麦V染色体引起的小麦-簇毛麦染色体代换系、易位系中线粒体蛋白质组的变化(简报)

THE EFFECT OF HAYNALDIA VILLOSA V CHROMOSOME ON THE MITOCHONDRIAL PROTEOME OF WHEAT- H. VILLOSA CHROMO SOME SUBSTITUTION LINE AND TRANSLOCATION lINE

线粒体是需氧生物中的一种半自主性细胞器，在能量代谢中，它起了一个关键的作用。柠檬酸循环、电子传递和氧化磷酸化过程均在线粒体内完成。线粒体具有高度的生物化学和遗传上的独立性，含有DNA和核糖核蛋白体，负责合成生物体内2％-5％的蛋白质。线粒体DNA具有自身复制能力，控制着众多的遗传性状，在植物中广泛存在的细胞质雄性不育则被认为是由线粒体基因组控制的性状。由于线粒体在能量代谢、遗传性状控制等多方面的功能，近5年中，线粒体蛋白质组的研究引起了研究者们的很大兴趣。

Wheat-Haynaldia villosa chromosome substitution line （6A/6V） and translocation lines （6DL/6VS, 6AL/6VS） were obtained through hybridization of H. villosa with powdery mildew susceptible cultivated wheat. Substitution line and translocation lines contain V chromosome or the chromosome short arm （VS） of H. villosa. They are resistant to powdery mildew. In this study, mitochondrial proteome changes were analyzed by using substitution line （6A/6V）, translocation line （6DL/6VS） as experimental materials in order to studying the effects of V chromosome on the mitochondrial proteome and related to powdery mildew resistance. The results indicated that 16 new mitochondrial protein spots （spot1, 22kDa/PI8.5; spot2, 31 kDa/PI 7.5; spot3, 28 kDa/PI 7.0; spot4, 31 kDa/PI 6.5; spot5, 40 kDa/PI 7.5; spot6,40 kDa/PI 7.4; spot7, 80 kDa/PI 8.4; spot8, 50 kDa/PI 7.5; spot9, 60 kDa/PI 7.3; spot10, 65 kDa/PI 6.6; spot11, 65 kDa/PI 6.6; spot12, 73 kDa/PI 7.5; spot13, 73 kDa/PI 7.7; spot14, 46 kDa/PI 7.4; spot15, 46 kDa/PI 7.3; spot16, 38 kDa/PI 6.3） were produced and 7 mitochondrial protein spots （spot1, 40 kDa/PI 7.5; spot2, 43 kDa/PI 7.6; spot3, 48 kDa/PI 7.5; spot4, 42 kDa/PI 8.0; spot5, 43 kDa/PI 7.5; spot6, 32 kDa/PI 4.8; spot7, 40 kDa/PI 5.5） were absent in substitution line, 7 new mitoehondrial protein spots （spot1, 43 kDa/PI 6.3; spot2, 60 kDa/PI 6.5; spot3, 60 kDa/PI 6.4; spot4,65 kDa/PI 7.5; spot5, 55 kDa/PI 8.2; spot6, 31 kDa/PI 8.0; spot7, 43 kDa/PI 8.0） were produced and 6 mitoehondrial protein spots （spot1＇, 66 kDa/PI 8.3; spot2＇, 58 kDa/PI 8.5; spot3＇, 36 kDa/PI 7.0; spot4＇, 48 kDa/PI 7.7; spot5＇, 48 kDa/PI 6.8; spot6＇, 43 kDa/PI 6.2） were absent in transloeation line. These experimental results suggest that V chromosome or VS of H. villosa can obviously lead mitoehondrial proteome changed. These changes may be associated with resistant to powdery mildew of substitution line and transloeation line.