壳多糖抗生素凝胶预防骨感染的实验研究

Experimental study of gentamicin chitosan gel for prevention of bone infection

目的：研究壳多糖庆大霉素凝胶对骨感染的预防作用。方法：按Ｎｏｒｄｅｎ法制作兔金黄色葡萄球菌骨髓炎模型，将５０只新西兰兔随机分为壳多糖庆大霉素组、壳多糖组、庆大霉素组、对照组。术后观察一般情况、Ｘ线片、骨培养及细菌计数。结果：壳多糖庆大霉素组上述指标优于其他各组，壳多糖组骨感染率无下降，但细菌计数等指标优于对照组，而庆大霉素组各观察指标和对照组无差别。结论：壳多糖庆大霉素凝胶能有效预防骨感染，可能与壳多糖具有抑菌作用、提高动物免疫机能、体内降解缓慢以及作为载体使抗生素有效浓度维持时间较长有关。

Objective:To study the efficacy of gentamicin chitosan gel in the prevention of bone infection. Methods: 50 New Zealand rabbits were randomly divided into 4 groups. According to the Norden technique, the model of osteomyelitis of the tuberosity of tibia was produced experimentally by injection of staphylococcal aurues, immediately following injection of gentamicin chitosan gel, chitosan gel, gentamicin as well as acetic acid solution as control. The severity of the infection was evaluated by clinical symptoms and signs, radiography, bone culture and bacterial counts. Results: All data showed significant improvement in the gentamicin chitosan group as compared to the others. Local injection of chitosan did not reduce the incidence of bone infection, however, it did provide statistical improvement when other data were concerned. There was no difference in all parameters between the gentamicin group and control. Conclusion: An injection of gentamicin chitosan gel is effective in preventing bone infection. The effects of gentamicin chitosan gel might be attributed to chitosan bacteriostasis, enhanced immunological activity, slow degradation within body as well as the ability as a carrier for sustained release of gentamicin.