人G-CSF重组表达系统稳定性的实验研究

Study of the stability of recombinant human G-CSF expression system

将构建好的人G－CSF表达工程菌（pJGW1-hGCSP／pGP1－2／DH5α），连续培养140代，其抗生素抗性无改变，重组质粒DNA酶切鉴定及目的蛋白表达率与原始苗基本一致。

The constructed engineering bacteria E. coli DH5α that contained plasmid pJGW1-hGCSF andpGP1-2 were cultured up to 140 generations. The antibiotics resistance and expressed protein level of rhG-CSF of the engineering bacteria is stable. The recombinant plasmids of pJGW1-hGCSF and pGP1 -2 of dif-ferent generations also showed the same electrophoretic patterns after restriction enzyme cleavage as thoseof the primary recombinant bacteria.