感染性心内膜炎瓣膜标本中肠道病毒RNA的检测

DETECT OF ENTEROVIRAL RNA IN THE HUMAN VALVE SPECIMENS WITH INFECTIVE ENDOCARDITIS

为探讨肠道病毒在感染性心内膜炎发病中的作用，应用逆转录聚合酶键反应对34例感染性心内膜炎患者的瓣膜标本和11树先心患者瓣膜（或心肌）标本进行肠道病毒基因检测。结果在感染性心内膜炎患者的瓣膜标本中阳性14例，阳性率41．2％；先心患者瓣膜（或心肌）标本阳性1例，阳性率9．1％，两者经统计学处理有显著性意义（P＜O．05）。结论：感染性心内膜炎的发病与肠道病毒感染有一定关系。

PURPOSE To make sure of the function of the enterovirus in infective endocarditis, 45 human valve specimens with infective endocarditis and congenital heart diseases were used to detect the enteroviral RNA in using the method of reversetranscription polymerase chain reaction (RT - PCR).METHODS All of the specimens were detected in using the method of RT - PCR.RESULTH Among 34 valve specimens with infective endocarditis, 14 cases were positive (positive ratio41. 2 % ). Among 11 valve specimens of congenital heart diseases, only one case was positive (Positive ratio9. 1 % ) (P<O.05).CONCLUSIONS The result showed that the enteroviral infection took an important role in the pathogenesis of infective endocarditis.