磁性化疗栓塞微球在大鼠肝癌中的导向治疗作用

Effect of targeting treatment with magnetic microspheres for live tumor in rats

目的：经肝动脉化疗栓塞已成为肝癌综合治疗的主要手段之一，为了最大限度地降低微球对癌外肝组织的损害，本研究采用磁性顺铂微球经肝癌大鼠肝动脉注入，观察其导向治疗作用。方法：首先将Ｗａｌｋｅｒ－２５６癌条接种于大鼠肝左叶被膜下，接种后７天肝癌大鼠随机分为４组，治疗组肝动脉注入磁性顺铂微球，肝癌部位的相应体表放置５２００Ｇ强磁场，并与肝动脉注入生理盐水、注入普通水剂顺铂、磁性顺铂微球体外不加磁场３个组进行比较。结果：治疗组的肝癌体积变化（Ｐ＜０．０１），肿瘤生长率（Ｐ＜０．０１），术后不同时期肝癌结节及正常肝组织中的铂的含量（Ｐ＜０．００１），ＳＧＰＴ含量（Ｐ＜０．０５）等观察指标缩小均显著优于其他各组。结论：磁性化疗栓塞微球在局部体外强磁场的导向作用下，经肝动脉注入后有着独特的导向治疗肝癌作用，且副作用小，值得临床应用。

Purpose: To maximize liver tumor drug uptake and minimize dose-limiting toxic effects in liver tissues of extra-tumor, Magetic Microspheres (MMS) with drugs were infused to the hepatic artery of liver tumor in rats, and evaluated the effect of targeting treatment. Method: Seven days after inocalation of walker-256 carcinosarcoma into the left lobe of liver of Sprague-Dawlay rats, normal conventional cisplatum MMS without externally magnetic field guiding MMS by an externally 5200G magnetic field guilding corresponded to liver tumor node at comparable dose were infused to the proper hepatic artery. Results: The results indicated that: the transplantable hepatocellular Carcinoma in rats were treated with MMS by an externally magnetic field gulding group had a significantly slower tumor growth (P<0.01), more extensive tumor necrosis, longer survival rate, higher platium levels in tumor node (P<0.001), and lower SGPT concentration (P<0.05) in comparsion with other three groups. Conclusions: It is suggested that MMS by an externally magnetic field guilding had safe and special effects of targeting chemocmbolization to liver tumor.