MTT法对膀胱肿瘤化疗药物敏感性的预测

MTT ASSAY AS A METHOD OF DETERMINING THE CHEMOSENSITIVITY IN HUMAN BLADDER CARCINOMA

①目的研究人膀胱肿瘤对化疗药物反应性，筛选对个体敏感的化疗药物。②方法采用四氮唑蓝（ＭＴＴ）法对手术切除的２７例膀胱肿瘤新鲜标本进行顺铂（ＤＤＰ）、保尔佳（ｐｏｌｙｅｒｇａ）、丝裂霉素（ＭＭＣ）、噻替哌（ＴＨＴ）、阿霉素（ＡＤＭ）和足叶乙甙（ＶＰ－１６）的抗癌药物敏感试验。③结果２７例中对３种药物敏感者１０例，占３７．０％；敏感药物顺序依次为：ＤＤＰ，ｐｏｌｙｅｒｇａ，ＭＭＣ，ＴＨＴ，ＡＤＭ和ＶＰ－１６，与临床治疗结果大致相符。④结论ＭＴＴ法可以作为化疗药物敏感试验的检测方法，为临床科学合理地选择用药提供依据。

Objective To study the reactivities of human bladder carcinoma to chemotherapeutic agents and screen the sensitive drugs for individuals. Methods The chemosensitivities of fresh bladder carcinoma samples from 27 patients to cisplatin,polyerga, mitomycin C, thiotepa,adriamycin and etoposide were determined by using a tetrazoliumbase decolorimetric assay(MTT assay). Results Ten cases(37.0%) were sensitive to 3 different drugs. They were DDP, polyerga, MMC, THT, ADM and VP16. Few patients were sensitive to other drugs. The order of senditivity to drugs was approximately in accordance with clinical curative effect. Conclusion The result may be used for clinical reference in selecting drugs.