肾上腺素对巨噬细胞吞噬和分泌功能的影响

The effects of epinephrine on phagocytosis and secretion functions of rat peritoneal macrophages

目的观察肾上腺素对巨噬细胞吞噬和分泌功能的影响。方法收集大鼠腹腔巨噬细胞，采用不同浓度肾上腺素（E）（2—100μ/L）刺激lh或10μg/L肾上腺素刺激不时间，检测细胞趋化、黏附、吞噬功能和培养上清TNF-α的浓度。结果低浓度肾上腺素处理时，巨噬细胞趋化、黏附、吞噬功能均明显增强，细胞分泌TNF-α明显增多，其中以10μg／L肾上腺素处理时细胞功能增强最明显。但是随着肾上腺素浓度进一步增加，巨噬细胞功能未见进一步增强。时效实验结果显示，不同时间处理（0．5—5．0）h可不同程度地增强巨噬细胞趋化、黏附，吞噬和分泌TNF-α的功能，其中以处理1h作用最明显。结论适度肾上腺素刺激可明显增强臣噬细胞免疫功能。

Objective To observe the modulatory effects of epinephrine （E） on the phagoeytosis and secretion funetlons of macrophages. Methods After collection of peritoneal macrophages from rats, the cells were stimulated for 1 h by ,apinephrine at different concentrations ranging from 2 μg/ L,or stimulated with 10 μg/L epinephrine for different durations. The immune functions of macrophages were evaluated,including chemotaxis, adherence,phagocytosis and the TNF-α levels in cell culture supernatants. Results When stimulated with epinephldno at low concentrations, the chemotaxis, aherence and phagocytic capacities of macrophages were enhanced obviously, and TNF-α secretion was improved significantly. The immune functions of macrophages ware enhanced mostly by 10 μg/L epinephrine. However, such functions of macrophage were not enhanced further more when treated by higher concentrations of epi- nephrine. Besides,the time-feeect experiments showed that within the treating time ranging from 0.5 h to 5.0 h by 10 p.g/L epinephrine, all of the chemotaxis,adherence, phagocytic capacities of macrophages and the TNF-α levels in cell culture supernts were improved to varying degrees, and the effects were the most after 1 h stimulation. Conclusion Acuts stimulation of the epinephrine at a suitable concentration can enhance the immune functions of macrophages.