ITP患儿血清可溶性IL-2受体水平和T淋巴细胞亚群变化

A preliminary study of serum sIL2R and Tlymphocyte subsets in children with idiopathic thrombopenic purpura

探讨特发性血小板减少性紫癜（ＩＴＰ）患儿免疫功能变化。方法采用双抗体夹心ＥＬＩＳＡ法对ＩＴＰ患儿进行了血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）测定和ＡＰＡＡＰ法检测Ｔ淋巴细胞亚群。结果ＩＴＰ患儿血清ｓＩＬ－２Ｒ、ＣＤ８显著升高，ＣＤ４／ＣＤ８显著下降（Ｐ＜０．０１），且ｓＩＬ－２Ｒ和ＣＤ４／ＣＤ８呈负相关（ｒ＝－０．５８１，Ｐ＜０．０１）；缓解期ｓＩＬ－２Ｒ明显下降，基本接近正常水平，而ＣＤ８、ＣＤ４／ＣＤ８比值较急性期变化不明显。

Objective We try to study the changes of immunological function in children with idiopathic thrompobpenic purpura (ITP). Methods The serum soluble interleukin2 receptor (sIL2R) levels and pcripheral blood Tlymphocyte subsets were detected by indirect ELISA and APAAP respectively. Results It showed that the levels of sIL2R and the percentage of CD8 were obviously higher in ITP than those in normal control(NC), the CD4/CD8 ratio was markedly lower in ITP than that in NC, and the serum sIL2R level correlated with the CD4/CD8 ratio. The sIL2R levels in children of convalescent stage were obviously decreased, but there were not much changes in the percentage of CD8 and CD4/CD8 ratio. Conclusions The results suggested that the aberrant sIL2R and Tlymphocyte subsets may involve in the pathogenesis of ITP.