电针不同穴组对抑郁大鼠行为活动及海马P-CREB表达的影响

Effects of electroacupuncture at different acupoint groups on behavior activity and p-CREB expression in hippocampus in the rat of depression

目的:观察不同穴组对抑郁症大鼠的疗效差异及其作用机制。方法:选用Wistar大鼠50只,随机分为正常组、模型组、体穴组、头穴组和百优解组。后4组用长期不可预见的中等强度刺激应激结合孤养的方法造成抑郁大鼠模型。模型组不给予任何治疗;百优解组每日按2 mg/kg灌胃给药1次;体穴组选取"太冲""内关""足三里",头穴组选取"百会""印堂""四神聪",2组均接电针仪,电针频率2 Hz,强度1 mA,每次20 min,每日1次。于实验前1 d和实验第7、14、21 d测量大鼠体重,并进行糖水消耗和强迫游泳试验,实验前1 d和实验第21 d进行开野实验。于第22 d观察各组海马p-CREB的表达。结果:与模型组相比,头穴组、百优解组的行为学评分、糖水消耗量均增加,游泳静止时间减少,海马的p-CREB阳性神经元数量增加(P<0.05);体穴组糖水消耗量增加,游泳静止时间减少(P<0.05),但行为学评分和海马的p-CREB阳性神经元数量增加不显著(P>0.05),各治疗组之间无显著差异(P>0.05)。结论:电针头穴体穴均能改善抑郁模型大鼠行为活动,有较好的抗抑郁作用。但头穴在增加模型大鼠体重、提高水平运动和垂直运动次数和提高海马p-CREB阳性神经元数量方面似乎优于体穴。

Objective To observe therapeutic effects and mechanisms at different acupoint groups for treatment of the depression rat. Methods Fifty Wistar rats were randomly divided into a normal group, a model group, a body acupoint group, a scalp acupoint group and a Fluoxetine hydrochloride group, 10 cases in each group. Depression rat model was established by using chronic unpredictable mild stress stimulation combined with lonely breeding for 21 days. EA （2 Hz, I mA, 20 min） was given at “Taichong” （LR 3）, “Neiguari” （PC 6） and “Zusanli” （ST 36） in the body point group and “Baihui” （GV 20）, “Yintang” （EX-HN 3）, “Sishencong” （EX-HN 1） in the head point group, once daily, and Fluoxetine hydrochloride was given to the Fluoxetine hydrochloride group by intragastric ad- ministration, 2 mg/kg, once daily, for 21 days. Body weight of the rat was recorded, and the sucrose-intake test and forced swimming test were conducted one day before the experiment and on the 7th, 14th and 21st day of the ex- periment, and the open field test was conducted one day before the experiment and on the 21st day. p-CREB expression in the hippocampus of the rats were observed on the 22nd day. Results As compared with the model group, in the head point＇group and the Fluoxetine group, the crossing and rearing movement times, the relative volume of sucrose-intake and p-CREB expression in the hippocampus increased significantly, and the duration of immovability in forced swimming test shortened significantly （P〈0.05）. The relative volume of sucrose-intake increased and the duration of immovability in forced swimming test in the body point group significantly shortened （P〈0. 05）, but the crossing and rearing movement times and p-CREB expression in the hippocampus did not insignificantly increase （P〉0. 05）, with no significant differences among the treatment groups （P〉0.05）. Conclusion EA at the head acupoints can improve behavioral activities and increase positive neuron number of p-CREB in the hippocampus of the depression rat, and selection of acupoints is of important role in treatment of depression.