降脂宁血中移行成分归属研究

Study on adscription of drug constituents in Beagle dog plasma after intragastric administration with Jiangzhining extract

目的:研究降脂宁(由制首乌、山楂、决明子和荷叶4味中药组成的复方制剂)Beagle犬灌胃给药后血中移行成分及其归属。方法:通过建立灌胃降脂宁后Beagle犬血浆HPLC图谱,比较降脂宁各单味药、给药及未给药Beagle犬血浆的HPLC图谱,结合色谱峰保留时间和紫外光谱分析,确认灌胃降脂宁后Beagle犬血中移行成分及其来源药味。结果:降脂宁给药后血浆中出现了24个移行成分,其中8个为降脂宁的原型成分,16个为代谢产物。结论:对降脂宁Beagle犬灌胃给药后血中移行成分及其代谢产物的研究有助于阐明其药效物质基础。

Objective： To investigate the drug constituents in Beagle dog plasma after intragastric administration with Jiangzhining extract, which is a Chinese medicine prescription and it consisting of processed Radix Polygoni Muhiflori, Fructus Crataegi, Semen Cassiae and Folium Nelumbinis. Method： The drug constituents and their origin in Beagle dogs plasma were assigned by comparison of high-performance liquid chromatographic profiles, retention time and ultraviolet spectra of the drug-free plasma, plasma contained drug, Jiangzhining extract and the ingredients of crude drug. Result： Twenty-four components were detected after intragastric administration of Jiangzhining extract, among which eight compounds were the original components existed in Jiangzhining extract and sixteen compounds were likely to metabolites of the some chemical constituents in ingredients of crude drug. Conclusion： Studies on the drug constituents and the metabolites in plasma after intragastric administration of Jiangzhining extract are useful to elucidate the effective substances of Jiangzhining prescription.