川芎内生真菌的分离与鉴定

Isolation and identification of endophytic fungi from Ligusticum

目的:探讨川芎内生真菌类群与川芎品种和产地的关系。方法:采用平板分离法分离川芎的内生真菌,采用点植法对分离菌株进行分类鉴定。结果:从6个产地的川芎根茎样品共获得内生真菌50株,经形态观察分类鉴定为1纲、3目、4科、13属。结论:不同产地及不同品种川芎的内生真菌在数量、分布、种群及其组成存在差异,推测川芎内生真菌种群可能和川芎的特定生境有关。

Objective： To investigate the correlation between entophytic fungal community, habitations and varieties of Ligusticum. Method： The solidified potato dextrose agar （PDA） of plates was applied for the isolation of the endophytic fungi, and the identification was completed by spot-planting method. Result： Fifty strains of the entophytic fungi were isolated from the rhizome of L. chuanxiong collected from 6 habitations. They were morphologically identified as belonging to 13 genera, 4 families, 3 orders and 1 class. Conclusion： There were some differences at quantity, species and distributing of the entophytic fungi in different habitats and varieties of Ligusticum, which was suggested that entophytic fungal community is related with specific habitat.