黄连提取物在大鼠肠外翻实验中的吸收研究

Absorption of extractive Rhizoma Coptidis in rat everted gut scas

目的:用肠外翻模型研究黄连提取物不同剂量在大鼠不同肠段的体外肠吸收特征。方法:采用大鼠肠外翻模型,以小檗碱(berberine,BER)和巴马汀(palmatine,PAL)为黄连提取物中代表成分,用HPLC对其进行检测,计算BER和PAL的吸收参数来分析其在肠道的吸收特征。结果:不同剂量时黄连提取物中BER和PAL在各肠段均为线性吸收,R2均大于0.9,符合零级吸收速率;BER和PAL的吸收速率常数(Ka)均随黄连提取物给药剂量的增加而增加(P<0.05),表明其为被动吸收。肠道不同部位的吸收实验表明,空肠对BER和PAL吸收有较为明显的优势,其次为回肠和结肠。结论:不同剂量黄连提取物中的BER和PAL在肠道不同部位吸收均符合零级吸收速率,其吸收形式可能为被动吸收。

Objective： Using in vitro everted gut scas to research the intestinal absorption of the extractive Rhizoma Coptidis at the different intestinal section and the different density. Method： Berberine （BER） and palmatine （PAL） which are representative compositions of the extractive Rhizoma Coptidis in everted gut scas are detected by HPLC, and calculated the absorption parameter to describe the character of absorption. Result： The absorption of BER and PAL is linearity in different intestine and different dose, and the square of coefficient correlation exceed 0. 9, which consistent with zero order rate process. The Ka of BER and PAL increases along with the raised dosage of the extractive Rhizoma Coptidis （ P 〈 0.05 ） , indicated it is the passive absorption. The absorption of BER and PAL in the jejunum is the most quick, the ileum and colon are slower. Conclusion： In the different dosage of extractive Rhizoma Coptidis, the absorption of BER and PAL Conforms to the zero order rate process at the different intestine, and is the passive absorption.