降糖消脂胶囊对2型糖尿病大鼠模型的胰岛及肝脏形态学改变的影响

Effect of Jiangtang Xiaozhi capsule on morphological changes of islet and liver in rat modle of type 2 diabtic mellitus

目的:观察降糖消脂胶囊对2型糖尿病大鼠模型的胰岛及肝脏形态学改变的影响,为临床治疗2型糖尿病提供试验依据。方法:采用高糖高脂饮食喂饲大鼠诱发胰岛素抵抗,按25 mg.kg-1体重2次腹腔注射链脲佐菌素(streptozotoc in,STZ)诱发高血糖症,造成2型糖尿病大鼠模型。将动物分为模型组、高、中、低剂量降糖消脂胶囊治疗组、阳性药二甲双胍组、阳性药金芪降糖片组和正常对照组,连续灌胃给药2个月后杀检,进行胰腺和肝脏形态学观察。结果:模型组和各治疗组均有不同程度的脂肪肝,其中降糖消脂中、低剂量组的肝脂肪变性明显轻于模型组(P<0.05)。降糖消脂高剂量组与模型组比较胰岛数明显增多,差异显著(P<0.05)。结论:降糖消脂胶囊能明显改善2型糖尿病大鼠模型的胰岛病变及肝细胞脂肪变性。

Objective： To explore the effect of Jiangtang Xiaozhi capsule （JXC） on morphological changes of islets and liver at rat model of type 2 diabetic mellitus and provide the experimental basis for the clinical therapy of type 2 diabetic mellitus. Method： Wister rats were fed on a diet enriched in fat and glucose to induce insulin resistan,the rats were injected intrapertoneally with a lowdose streptozotocin （STZ） twice （25 mg · kg^-1） to induce hyperglycemia, so the successful rat model of type 2 diabetes were established. The experimental rats were divided into model group, high dose JXC group, middle dose JXC group, low dose JXC group, Erjiashuanggua group, Jinqijiangtang group and normal control group. After all the treatment groups received their own medicine for two months, all the rats were sacrificed and morphological examination on their islets and livers were performed. Result： Fatty liver in various degrees was seen in the model group and all the treatment groups, but the liver steatosis in middle and low dose JXC groups was significantly milder than that in model group （ P 〈 0. 05 ）. Islets in the high dose JXC group were significantly more than that in the model group （ P 〈 0. 05）. Conclusion： JXC can improve significantly the pathological change in islets and liver steatosis at rat model of type 2 diabetic mellitus.