六君子加减方对大鼠胃溃疡黏膜组织生长因子的影响

Research of Chinese drugs Liujunzi formula on EGF,bFGF of gastric mucous in rats

[目的]观察六君子加减方对溃疡大鼠胃黏膜表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响,进一步探讨其可能的作用机制。[方法]32只雄性Waistar大鼠,8只作为正常对照(正常)组,24只采用Okabe乙酸烧灼法在大鼠胃前壁造成溃疡模型,随机分为模型对照(模型)组、六君子加减方(六君子方)组、硫糖铝组,每组8只,各组分别连续给药治疗10 d。给药结束后,定量检测溃疡周围组织EGF和bFGF的水平。[结果]模型组大鼠胃组织EGF及bFGF的水平明显高于正常组(P<0.01);六君子方组胃组织EGF及bFGF的水平低于模型组(P<0.05),而与正常组和硫糖铝组比较,差异均无统计学意义(P>0.05)。[结论]中药六君子加减方可能通过对胃组织EGF及bFGF水平的调控而促进损伤后黏膜的愈合,可能是其作用机制之一。

[Objective]To observe the effect of Chinese drugs of Liujunzi formula on EGF and bFGF of gastric mucous in rats and illustrate its mechanism of protecting the gastric mucous.[Methods]The gastric ulcer model was established by dropping acetic acid on the rat＇s surface of gastric mucosa. Rats were randomly divided into 4 groups, normal group, model group, herbs group, sucralfate group. All of these rats were taken different medicine in 10 days. When this time ended, the content of EGF,bFGF in gastric tissue were observed rationally. [Results] EGF and bFGF content in mucosa in model group was higher than that in the normal group （ P〈 0. 01） ; EGF and bFGF content in mucosa in Liujunzi formula group was lower than that in the model group （ P〈0. 01） and had no difference among the normal group ,the Liujunzi formula group and the sucralfate group （P〉0.05）. [Conclusion ]Liujunzi formula can decrease EGF and bFGF content in gastric mucous of gastric tissue, and that may be one of mechanism of protecting the gastric mucous.